Protein Info for SMc02585 in Sinorhizobium meliloti 1021

Annotation: sensor histidine kinase transmembrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 transmembrane" amino acids 23 to 43 (21 residues), see Phobius details amino acids 48 to 69 (22 residues), see Phobius details amino acids 80 to 116 (37 residues), see Phobius details amino acids 127 to 148 (22 residues), see Phobius details amino acids 159 to 181 (23 residues), see Phobius details PF00512: HisKA" amino acids 212 to 272 (61 residues), 36.6 bits, see alignment E=3.7e-13 PF02518: HATPase_c" amino acids 324 to 424 (101 residues), 66.2 bits, see alignment E=3.6e-22

Best Hits

Swiss-Prot: 41% identical to REGB_RHOS4: Sensor histidine kinase RegB (regB) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

KEGG orthology group: K15011, two-component system, sensor histidine kinase RegB [EC: 2.7.13.3] (inferred from 100% identity to sme:SMc02585)

Predicted SEED Role

"Sensor histidine kinase PrrB (RegB) (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92TA1 at UniProt or InterPro

Protein Sequence (433 amino acids)

>SMc02585 sensor histidine kinase transmembrane protein (Sinorhizobium meliloti 1021)
MAAATLYNDLNSNRSLRLQTLVRLRWLAVGGQSLAVIVTALWLQFPLPVVPCSVLIACLA
LLNVFLTLRFPPTQRLTPPAAFTLLGIDLAQLTALLFITGGLANPFAPLLCVPVIISSAS
QPKPQSIVLAGLAVLGVTALAFSPFPLPWYPGTLLLMPRVLTAGIWFAIVSMTAFAAFYT
YRVSLEASELSEALTATELVLQREKHLSQLDGLAAAAAHELGTPLATISVVAKEMERELG
DDPRFGEDVHLLRSQSERCRDILRRLTTLSSESEEHMRLLPLSSLIEEVMAPHREFGIEI
ELKEQGDRASEPVGIRNAGILYGLGNLLENAVDYARKKVTVTTEHTAERVRVTIEDDGDG
FSPDILARIGEPYVTRRQKDDSAGGLGLGLFIAKTLLERSGARLRFENGGSKHPGARVSV
EWPRVLMDTKLAK