Protein Info for SMc02499 in Sinorhizobium meliloti 1021

Annotation: F0F1 ATP synthase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 509 TIGR00962: ATP synthase F1, alpha subunit" amino acids 3 to 509 (507 residues), 828.8 bits, see alignment E=8.3e-254 PF02874: ATP-synt_ab_N" amino acids 25 to 92 (68 residues), 72.7 bits, see alignment E=4.3e-24 PF00006: ATP-synt_ab" amino acids 149 to 372 (224 residues), 271.2 bits, see alignment E=9.3e-85 PF00306: ATP-synt_ab_C" amino acids 379 to 504 (126 residues), 167 bits, see alignment E=3.6e-53

Best Hits

Swiss-Prot: 100% identical to ATPA_RHIME: ATP synthase subunit alpha (atpA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K02111, F-type H+-transporting ATPase subunit alpha [EC: 3.6.3.14] (inferred from 100% identity to smk:Sinme_3089)

MetaCyc: 72% identical to ATP synthase subunit alpha, mitochondrial (Homo sapiens)

Predicted SEED Role

"ATP synthase alpha chain (EC 3.6.3.14)" in subsystem F0F1-type ATP synthase (EC 3.6.3.14)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92LK6 at UniProt or InterPro

Protein Sequence (509 amino acids)

>SMc02499 F0F1 ATP synthase subunit alpha (Sinorhizobium meliloti 1021)
MDIRAAEISAILKDQIKNFGQEAEVSEVGQVLSVGDGIARVYGLDNVQAGEMVEFPGGIR
GMALNLEADNVGVVIFGSDRDIKEGDTVKRTGAIVDVPVGPELLGRVVDALGNPIDGKGP
INAKQRARVDVKAPGIIPRKSVHEPMSTGLKAIDALIPVGRGQRELVIGDRQTGKTAIIL
DTILNQKAIHDNGPEGDKLYCVYVAIGQKRSTVAQFVKVLEERGALQYSIIVAATASDPA
PMQYLAPFAGCAMGEYFRDNGKHALIGYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFY
LHSRLLERAAKLSDERGAGSLTALPVIETQGNDVSAFIPTNVISITDGQIFLETDLFYQG
IRPAVNVGLSVSRVGSSAQIKAMKQVAGSIKGELAQYREMAAFAQFGSDLDAATQRLLNR
GARLTELLKQPQFSPLKTEEQVAVIFAGVNGYLDKLPVSDVGKFEHGLLSYLRSEGKAVL
DTIRTEKAISDDTKAKLKGAIDSFAKSFA