Protein Info for SMc02482 in Sinorhizobium meliloti 1021

Annotation: 2-oxoglutarate dehydrogenase E1 component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 998 PF16078: 2-oxogl_dehyd_N" amino acids 13 to 51 (39 residues), 61.7 bits, see alignment (E = 7.5e-21) TIGR00239: oxoglutarate dehydrogenase (succinyl-transferring), E1 component" amino acids 111 to 996 (886 residues), 1138.7 bits, see alignment E=0 PF00676: E1_dh" amino acids 250 to 574 (325 residues), 194.3 bits, see alignment E=5e-61 PF02779: Transket_pyr" amino acids 643 to 836 (194 residues), 173.8 bits, see alignment E=5.4e-55 PF16870: OxoGdeHyase_C" amino acids 841 to 996 (156 residues), 181.9 bits, see alignment E=1.3e-57

Best Hits

Swiss-Prot: 81% identical to ODO1_OCHA4: 2-oxoglutarate dehydrogenase E1 component (sucA) from Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / NBRC 15819 / NCTC 12168)

KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 90% identity to agr:AGROH133_08852)

Predicted SEED Role

"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7APA7 at UniProt or InterPro

Protein Sequence (998 amino acids)

>SMc02482 2-oxoglutarate dehydrogenase E1 component (Sinorhizobium meliloti 1021)
MTRQEANEQFQLTSFLDGANAAYIEQLHARYEADPSSVSAEWQSFFKALADRPEDVVRAA
KGASWKKQNWPIPANGELVSALDGDWGTVEKVIGKKVKAKAEEAAAVAGVALSEAEVHQS
TRDSVRAIMMIRAYRMRGHLHAKLDPLGLADPVEDYDELSPKTYGFEEKDYDRKIFIDNV
LGLEYATVREMVEILERTYCSTIGVEFMHMSNPEEKGWIQERIEGPDKGVEFTPEGKKAI
LQKLIEAEGFEQFIDVKYKGTKRFGVDGGESLIPALEQIIKRGGQLGLKEIVLGMAHRGR
LNVLSQVMAKPHRAIFHEFKGGSYTPDDVEGSGDVKYHLGASSDREFDGNKVHLSLTANP
SHLEIVNPVVMGKARAKQDQMATVFEGDIIPLRERVKVMPLILHGDAAFAGQGVVAEILG
LSGLRGHRVGGTVHFIINNQIGFTTNPAFSRSSPYPSDVAKMIEAPIFHVNGDDPEAVVY
AAKVATEFRMKFHKPVVIDMFCYRRFGHNEGDEPAFTQPKMYKAIRAHKTVVQLYSDRLI
AEGLISEGEVEKMKADWRAHLEQEFEAGQSYKPNKADWLDGAWSGLRTADNQDEQRRGRT
SVPMKQLKEVGRKLSEIPAGFSAHRTIQRFMENRANMIQTGEGIDWAMAEALAFGTLVTE
GTKIRLSGQDCERGTFSQRHSVLYDQETEERYIPLANLSPTQARYEVINSMLSEEAVLGF
EYGYSLARPNALTLWEAQFGDFANGAQVVFDQFISSGERKWLRMSGLVCLLPHGYEGQGP
EHSSARLERFLQLCAEDNMQVANVTTPANYFHILRRQVKRDFRKPLILMTPKSLLRHKRA
VSSLSEMAGESSFHRLLWDDAEVIKDGPIKLQKDSKIRRVVMCSGKVYYDLLEEREKRGI
DDIYLLRVEQLYPFPAKALINELSRFRNAEMVWCQEEPKNMGAWSFIDPYLEWVLAHIDA
KYQRVRYTGRPAAASPATGLMSKHLAQLAAFLEDALGG