Protein Info for SMc02436 in Sinorhizobium meliloti 1021

Annotation: peptide chain release factor 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 360 TIGR00019: peptide chain release factor 1" amino acids 7 to 354 (348 residues), 503.3 bits, see alignment E=1.8e-155 PF03462: PCRF" amino acids 13 to 204 (192 residues), 238.7 bits, see alignment E=4.9e-75 PF00472: RF-1" amino acids 211 to 320 (110 residues), 144.9 bits, see alignment E=1e-46

Best Hits

Swiss-Prot: 100% identical to RF1_RHIME: Peptide chain release factor 1 (prfA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K02835, peptide chain release factor 1 (inferred from 100% identity to smk:Sinme_2666)

Predicted SEED Role

"Peptide chain release factor 1" in subsystem LMPTP YwlE cluster

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92MK5 at UniProt or InterPro

Protein Sequence (360 amino acids)

>SMc02436 peptide chain release factor 1 (Sinorhizobium meliloti 1021)
MAKLPVEKMRELERRFGEIEARMSAGPSADVYVKLASEYSELQPVVSKIRAYEKATAELA
DIAAMLADRATDKDMRDLAEMEKPEVEEKIEALEQEIQILLLPKDAADEKSAILEIRAGT
GGSEAALFAGDLFRMYERYAASKGWRVEVLSASEGEAGGYKEIIATVSGRGVFSRLKFES
GVHRVQRVPETEASGRIHTSAATVAVLPEAEEIDIDVRPEDIRVDTMRSSGAGGQHVNTT
DSAVRITHIPTGIVVTSSEKSQHQNRAKAMQVLRSRLYDMERQRADSERSADRRSQVGSG
DRSERIRTYNFPQGRLTDHRINLTLYKLDRMMEGEIDDVVDALLADYQASQLALLGERQN