Protein Info for SMc02053 in Sinorhizobium meliloti 1021

Annotation: tRNA (uracil-5-)-methyltransferase Gid

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 472 TIGR00137: tRNA:m(5)U-54 methyltransferase" amino acids 10 to 467 (458 residues), 535.8 bits, see alignment E=3.7e-165 PF01134: GIDA" amino acids 11 to 381 (371 residues), 325.6 bits, see alignment E=2.5e-101

Best Hits

Swiss-Prot: 100% identical to TRMFO_RHIME: Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO (trmFO) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K04094, glucose inhibited division protein Gid (inferred from 100% identity to smk:Sinme_1345)

Predicted SEED Role

"tRNA:m(5)U-54 MTase gid" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92Q15 at UniProt or InterPro

Protein Sequence (472 amino acids)

>SMc02053 tRNA (uracil-5-)-methyltransferase Gid (Sinorhizobium meliloti 1021)
MNKTTSSHSPIHVIGGGLAGSEAAWQIAEAGVPVILHEMRGVRGTDAHKTESLAELVCSN
SFRSDDATSNAVGVLHAEMRLAGSLIMRCADLNQVPAGGALAVDREGFAEAVSAAIADHP
LITVLREEIRGLPPKDWDLAIIATGPLTAPDLAEAIRAETGADALAFFDAIAPIVHADTI
DMDICWHQSRYDKVGPGGTGKDYINCPLTQEQYDAFVDALIAGDKAGFKEWEGTPYFDGC
LPIEVMAERGRETLRHGPMKPMGLTNAHNPSVKPYAVVQLRQDNALGTLYNMVGFQTKLK
YGAQGEVFRMIPGLQNAEFARLGGLHRNTYINSPTLLDHSLTLKSRPGLRFAGQITGCEG
YVESASIGLLAGRFAAAERKGVSPVLPPVTTAFGALLDHITGGHIVSDDEPGKRSFQPMN
INFGLFPPLEPGALTRPEGAKRFRGKEKALAKKQAMASRALSDCASWLDQAV