Protein Info for SMc01934 in Sinorhizobium meliloti 1021

Annotation: prolyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 442 TIGR00409: proline--tRNA ligase" amino acids 1 to 224 (224 residues), 328.9 bits, see alignment E=3.8e-102 PF00587: tRNA-synt_2b" amino acids 96 to 330 (235 residues), 90.1 bits, see alignment E=1.8e-29 PF03129: HGTP_anticodon" amino acids 348 to 439 (92 residues), 81.9 bits, see alignment E=3.2e-27

Best Hits

Swiss-Prot: 100% identical to SYP_RHIME: Proline--tRNA ligase (proS) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 100% identity to smk:Sinme_1082)

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92QN2 at UniProt or InterPro

Protein Sequence (442 amino acids)

>SMc01934 prolyl-tRNA synthetase (Sinorhizobium meliloti 1021)
MRLSRFFMPILKENPKEAEIVSHRLMLRTGMVRQQSAGIYTWLPLGKRVLDKVNAIIREE
QNRSGAIELLMPTLQSAELWQESGRYDAYGKEMLRIKDRQDRPMLYGPTNEEMITDIFRS
YVKSYRNLPLNLYHIQLKFRDEIRPRFGTMRSREFLMKDAYSFDLDRAGAEHAYNKMFAA
YLRTFDRMGLRAIPMRADTGPIGGNLSHEFIILADTGESEVFCHKDFLGFDIPGEDTNFD
DVAGLKTIFDKWTSRYAATSEMHDEAAFGAIAEGERLSARGIEVGHIFYFGTKYSEAMGA
KVLGPDGKEHTVHMGSYGIGPTRLVPAIIEASHDDNGIIWPKGIAPFDVVVINMKTGDEA
CDAACGKVYSDLGKAGFDVLLDDTDERAGGKFATADLIGVPVQVIVGPRSIANGEVEVKD
RKTGARETMTVEAAINKLVAAR