Protein Info for SMc01461 in Sinorhizobium meliloti 1021

Annotation: DNA helicase II protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 857 PF13604: AAA_30" amino acids 47 to 113 (67 residues), 27.3 bits, see alignment 8.4e-10 PF00580: UvrD-helicase" amino acids 47 to 281 (235 residues), 244.9 bits, see alignment E=3.8e-76 amino acids 328 to 359 (32 residues), 42.5 bits, see alignment (E = 2.1e-14) PF13245: AAA_19" amino acids 51 to 279 (229 residues), 62.1 bits, see alignment E=1.9e-20 PF13361: UvrD_C" amino acids 364 to 694 (331 residues), 220.7 bits, see alignment E=1.2e-68

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 77% identity to bcs:BCAN_A1446)

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92NP4 at UniProt or InterPro

Protein Sequence (857 amino acids)

>SMc01461 DNA helicase II protein (Sinorhizobium meliloti 1021)
MTKGFDDIPFFDEEPAPRKPAAADGGIAARAMAARDKAQRPDYVSGLNPEQREAVEALEG
PVLVLAGAGTGKTRVLTTRIAHILSTGRAYPSQILAVTFTNKAAREMKERIGVLVGHAVE
GMPWLGTFHSIGVKLLRRHAELVGLRSDFTILDTDDVVRLIKQLIQAEGLDDKRWPAKQF
AGMIDTWKNKGLDPSQIPEGDARAFANGKGRELYAAYQNRLLTLNACDFGDLLLHPIRMF
RANPDVLKEYHAKFRYILVDEYQDTNTAQYMWLRLLAQQTQASRNRPSSGLPATFSPSDG
EKGQAARSQSPLSPSERGEGRGEGQPTVNICCVGDDDQSIYGWRGAEVDNILRFEKDFPG
AKVIKLERNYRSTEHILGAAAHLIAHNEGRLGKTLFTERTNPDDEKVHVHAAWDSEEEAR
AIGEEIEQLQRKKHNLNDISILVRASFQMREFEDRFVTLGLNYRVIGGPRFYERLEIRDA
MAYFRLVCQPADDLAFERIVNTPKRGLGETTVRTLHDYARARDIPMLAAASDIVETDELK
PKARKGLFDVVADFRRWQTLLETTPHTELAERILDESGYTAMWQADKSAEAPGRLENLKE
LIRSMEAFESMRGFLEHVALVMDAEQNENMDAVSIMTLHSAKGLEFDTVFLPGWEEGLFP
HQRALDEGGRAGLEEERRLAYVGITRAKRRCHIWFVSNRRIHGLWQSTLPSRFLDELPIA
HVEVAEQEVSYGGYGRGGYGQSRFDKADPFENNYQTPGWKRAQQHRSEATRDNWGTRSGH
AIERIGYGESGPRTRTIEGELVAKSTSAEPSRFNVGDRVFHIKFGNGNIAAIEGNKLTID
FDRAGQKRVLDGFVERV