Protein Info for SMc01237 in Sinorhizobium meliloti 1021

Annotation: ribonucleotide-diphosphate reductase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08471: Ribonuc_red_2_N
  • amino acids 31 to 147 (117 residues), 143.5 bits, E=2.1e-46
• TIGR02504: ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent
  • amino acids 126 to 753 (628 residues), 468.5 bits, E=1.7e-144
• PF02867: Ribonuc_red_lgC
  • amino acids 198 to 752 (555 residues), 348.7 bits, E=5.8e-108
  • amino acids 809 to 913 (105 residues), 57.1 bits, E=1.4e-19

Best Hits

Swiss-Prot: 88% identical to NRDJ_AGRFC: Vitamin B12-dependent ribonucleotide reductase (nrdJ) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 88% identity to atu:Atu1733)

Predicted SEED Role

"Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (7/8 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (3/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (3/4 steps found)

KEGG Metabolic Maps

• Glutathione metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.4.1

Sequence Analysis Tools

See Q92Q05 at UniProt or InterPro

Protein Sequence (1263 amino acids)

>SMc01237 ribonucleotide-diphosphate reductase subunit alpha (Sinorhizobium meliloti 1021)
MKIERRFTKAGQSAYAEIEFRKATSEIKNPDGSVVFRLADIDVPAQFSQVAADILAQKYF
RKAGVPAKLKRVEENDVPSFLWRSVPDNDALKALPKEEQYGSETDARQVFDRLAGTWAYW
GWKGGYFDTEEDASAFRDELAYMLATQRVAPNSPQWFNTGLHWAYGIDGPGQGHFYVDPF
TGKLTKSKSAYEHPQPHACFIQSVADDLVNEGGIMDLWVREARLFKYGSGTGSNFSHLRA
EGERLSGGGKSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVVVDIDHPDIEEYINWKVKE
EQKVAALVTGSKIVAKHLKAIMKACINCDGSDDACFDPKQNPALKREIRAAKQALVPENY
VKRVIQFARQGYKDIQFKTYDTDWDSEAYLTVSGQNSNNSVSIKDDFLRAVEADGDWHLT
ARKDGRVMKTLKARDLWESISHAAWASADPGLHFNTTMNDWHTCPAAGPIRASNPCSEYM
FLDDTACNLASLNLMQFKDAATKRINIGDYEHAVRLWTVVLEVSVMMAQFPSREIAELSY
EYRTLGLGYANIGGLLMSSGIPYDSPEGRAIAGALTAIMTGVAYATSAEISAKLGPFPGF
APNRDNMLRVMRNHRRAAHGETAGYEGLSVNPVALVHGDCPDQDLIAHAKGAWDKAVELG
EKHGYRNAQATVIAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKIINRAVPEALR
TLGYSESQIAEMEAYAVGHGNLNQAPAINPTTLKAKGFTDEKIEAVNAALKAAFDIKFVF
NQWTLGADFLKETLKVSDEQLASMDFNLLEHLGFSRKDIEAANIHVCGAMTLEGAPFLKA
EHLPVFDCANPCGKIGKRYLSVESHIRMMAAAQPFISGAISKTINMPNEATVEDCKNAYM
LSWKLALKANALYRDGSKLSQPLNASLIEDEEDEDAIEELMQAPAAAQAVAVTEKIIERV
IEKVVRAREKLPNRRQGYTQKAIVGGHKVYLRTGEFGDGRLGEIFIDMHKEGAAFRAMMN
NFAIAISLGLQYGVPLEEYVEAFTFTKFEPAGMVQGNDAIKNATSILDYVFRELAVSYLG
RHDLAHVDTSDFSNTALGRGIQEGKTNLVSTGWTRGYKPSIVGGTGERSEPKGASTAAPA
RASAGASVTSISGNAVRKLEPAIAAATSEVVAFKRDYEERAVELAEEIAEEAEQEVTALF
SDKAAAEAAAAKSEAKKVEAERRARSIMQGYTGNMCSECQNFTMVRNGTCEKCDTCGATS
GCS