Protein Info for SMc01010 in Sinorhizobium meliloti 1021

Annotation: threonyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 661 PF02824: TGS" amino acids 9 to 58 (50 residues), 30.7 bits, see alignment 5.2e-11 TIGR00418: threonine--tRNA ligase" amino acids 75 to 645 (571 residues), 676.5 bits, see alignment E=2e-207 PF07973: tRNA_SAD" amino acids 173 to 222 (50 residues), 52.5 bits, see alignment 7.7e-18 PF00587: tRNA-synt_2b" amino acids 329 to 543 (215 residues), 171 bits, see alignment E=5.7e-54 PF03129: HGTP_anticodon" amino acids 555 to 643 (89 residues), 73.6 bits, see alignment E=2.3e-24

Best Hits

Swiss-Prot: 100% identical to SYT_RHIME: Threonine--tRNA ligase (thrS) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 100% identity to sme:SMc01010)

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92QB0 at UniProt or InterPro

Protein Sequence (661 amino acids)

>SMc01010 threonyl-tRNA synthetase (Sinorhizobium meliloti 1021)
MMSHSVSLTFPDGSVREFAAGTTGRDVAESISKSLAKKAVAIAISGELRDLSDAVTEGRI
EIVTREDKRALELIRHDAAHVMAEAVQELWPGTQVTIGPVIDNGFYYDFAKNEPFTPDDL
PVIEKKMREIIARNKPFTKEVWSRDRAKEVFAAKGENYKVELVDAIPEGQDLKIYYQGDW
FDLCRGPHMASTGQIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAWHTQEELDQYLHVLA
EAEKRDHRRLGREMDLFHFQEEGPGVVFWHGKGWRIFQSLVAYMRRRLEGDYQEVNAPQV
LDKSLWETSGHWGWYRDNMFKVTVAGDDTDDDRVFALKPMNCPGHIQIFKHGLKSYRELP
VRLAEFGAVHRYEPSGALHGLMRVRGFTQDDAHIFCTDEQMAAECLKINDLILSVYEDFG
FKEIVVKLSTRPEKRVGSDELWDRAEAVMTEVLQTIEEQSEGRIKTGILPGEGAFYGPKF
EYTLKDAIGREWQCGTTQVDFNLPERFGAFYIDSESEKRQPVMIHRAICGSMERFLGILL
ENYAGHMPLWISPLQVVVATITSEADDYGREVAERLRDAGLTVETDFRNEKINYKVREHS
VTKVPVIVVCGRREAEERSVNIRRLGSQAQTAMSLDEAVASLSAEAMAPDLKRRSNSRKS
V