Protein Info for SMc00561 in Sinorhizobium meliloti 1021

Annotation: replicative DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 TIGR00665: replicative DNA helicase" amino acids 23 to 490 (468 residues), 507 bits, see alignment E=2.1e-156 PF00772: DnaB" amino acids 23 to 124 (102 residues), 92.3 bits, see alignment E=2.6e-30 PF03796: DnaB_C" amino acids 201 to 488 (288 residues), 341.7 bits, see alignment E=3.8e-106 PF13481: AAA_25" amino acids 216 to 393 (178 residues), 54.7 bits, see alignment E=1.6e-18

Best Hits

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 100% identity to smk:Sinme_0927)

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92R03 at UniProt or InterPro

Protein Sequence (499 amino acids)

>SMc00561 replicative DNA helicase (Sinorhizobium meliloti 1021)
MNDAARKLAPLAKDQAEQHYREAPNNLEAEQALLGAILVNNDAFYRVSDFLKPVHLYEPL
HRRIFEIAGEIIRMGKTANPVTVKTFLKADEKVGDLTVAQYLARLAAEAVSIINAEDYGR
AIYDLALRRSLITIGEDMVNIAYDAPLDMPPQSQIEDAERRLFELAETGRYDGGFQSFND
AVALAIDMAGQAFERDGHLSGISTGIHSLDGKMGGLQRSDLIILAGRPGMGKTSLATNIA
YNIAAAYEPEVQPDGSFKAKNGGVVGFYSLEMSSEQLATRIISEQTEVSSSKIRRGDISE
ADFEKLVACSQMMQKVPLYIDQTGGISIAQLAARARRLKRQRGLDVLVVDYIQLMTGSKK
SGENRVQEITEITTGLKALGKELNVPIIALSQLSRAVESREDKRPQLSDLRESGSIEQDA
DVVLFVFREEYYVKNMEPRDEFDPKYEEWKMQMEKVKGTADVIIAKQRHGPTGTVKLAFQ
SEFTRFSDLADPSFTQYEH