Protein Info for SMc00127 in Sinorhizobium meliloti 1021

Annotation: choline sulfatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 512 TIGR03417: choline-sulfatase" amino acids 5 to 509 (505 residues), 897.9 bits, see alignment E=1e-274 PF00884: Sulfatase" amino acids 6 to 352 (347 residues), 136.8 bits, see alignment E=2.1e-43 PF01663: Phosphodiest" amino acids 9 to 298 (290 residues), 35.4 bits, see alignment E=1.9e-12 PF16347: SGSH_C" amino acids 302 to 430 (129 residues), 43.3 bits, see alignment E=9.4e-15 PF12411: Choline_sulf_C" amino acids 457 to 508 (52 residues), 72.7 bits, see alignment 3.2e-24

Best Hits

Swiss-Prot: 100% identical to BETC_RHIME: Choline-sulfatase (betC) from Rhizobium meliloti (strain 1021)

KEGG orthology group: None (inferred from 100% identity to smk:Sinme_0659)

MetaCyc: 100% identical to choline sulfatase (Sinorhizobium meliloti Rm2011)
Choline-sulfatase. [EC: 3.1.6.6]

Predicted SEED Role

"Choline-sulfatase (EC 3.1.6.6)" in subsystem Choline Transport or Choline and Betaine Uptake and Betaine Biosynthesis (EC 3.1.6.6)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.6.6

Use Curated BLAST to search for 3.1.6.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See O69787 at UniProt or InterPro

Protein Sequence (512 amino acids)

>SMc00127 choline sulfatase (Sinorhizobium meliloti 1021)
MTTGKPNILIIMVDQLNGKLFPDGPADFLHAPNLKALAKRSARFHNNYTSSPLCAPARAS
FMAGQLPSRTRVYDNAAEYQSSIPTYAHHLRRAGYYTALSGKMHFVGPDQLHGFEERLTT
DIYPADFGWTPDYRKPGERIDWWYHNLGSVTGAGVAEITNQMEYDDEVAFLANQKLYQLS
RENDDESRRPWCLTVSFTHPHDPYVARRKFWDLYEDCEHLTPEVGAIPLDEQDPHSQRIM
LSCDYQNFDVTEENVRRSRRAYFANISYLDEKVGELIDTLTRTRMLDDTLILFCSDHGDM
LGERGLWFKMNFFEGSARVPLMIAGPGIAPGLHLTPTSNLDVTPTLADLAGISLEEVRPW
TDGVSLVPMVNGVERTEPVLMEYAAEASYAPLVAIREGKWKYVYCALDPEQLFDLEADPL
ELTNLAENPRGPVDQATLTAFRDMRAAHWDMEAFDAAVRESQARRWVVYEALRNGAYYPW
DHQPLQKASERYMRNHMNLDTLEESKRYPRGE