Protein Info for SMa1573 in Sinorhizobium meliloti 1021

Annotation: CpaE2 pilus assembly protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 586 transmembrane" amino acids 451 to 472 (22 residues), see Phobius details PF13614: AAA_31" amino acids 162 to 325 (164 residues), 47.2 bits, see alignment E=5.2e-16 PF06564: CBP_BcsQ" amino acids 163 to 309 (147 residues), 31.2 bits, see alignment E=3.4e-11 PF01656: CbiA" amino acids 164 to 341 (178 residues), 27.8 bits, see alignment E=4.4e-10 PF07811: TadE" amino acids 445 to 486 (42 residues), 38.8 bits, see alignment 1.7e-13

Best Hits

KEGG orthology group: K02282, pilus assembly protein CpaE (inferred from 100% identity to sme:SMa1573)

Predicted SEED Role

"Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly" in subsystem Widespread colonization island

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92YL8 at UniProt or InterPro

Protein Sequence (586 amino acids)

>SMa1573 CpaE2 pilus assembly protein (Sinorhizobium meliloti 1021)
MEIPNMKQLDSETREAPQPMTAAPLLPIPKVDIAVFCQSEEVREAVGTAAIDRRMARATV
TVKAGGMKEATALYGGVTSPNLVVVESDDGEARLMATLETLAMECVTGTKVIVIGRSNDV
GLYKKLLDAGVSDYLVKPLEPMDFVAAVHRCFRDSTEEKLGRIVAFVGAKGGTGSSTLAH
NVAYAMSKRVDADVLLADLDLQSGTLGLNFDIEAKHGMVDVLQSPDRLDDVLLRRLAVSY
TDRLHLLPATTDLDKFINLREGDVDHLLDVARSSSWHVVVDLPHILTQWTRKILLEADEI
VVTATPDLAGMRNAKNLIDFLKKARPNDPPPRLVLNKVGTPKLQEIKPKDFVAAVGLEEG
VSLAFEPSLFGAAANNGRLVIESAPDSKAGKAIVSLAWRVGGTRERRTRQKGVKALLQKV
FKRGKPKAPSTLRKKAGELKTSEAGASAVEFALVAPVLALGLVATADLGLAIHERMTIDH
VLRAGAQAALADPGAAQVQKVLVSTLAESPRLASAVLPAVKRYCACPENADVAPEAAPQC
GTVTCANAKPQFVYYRLAAAKSYRPMSLPAVLPVFELGSSMQVQVQ