Protein Info for SMa1529 in Sinorhizobium meliloti 1021

Annotation: NuoD2 NADH I chain D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01962: NADH dehydrogenase (quinone), D subunit
  • amino acids 19 to 404 (386 residues), 632 bits, E=1.7e-194
• PF00346: Complex1_49kDa
  • amino acids 133 to 404 (272 residues), 392.2 bits, E=5.1e-122

Best Hits

Swiss-Prot: 100% identical to NUOD2_RHIME: NADH-quinone oxidoreductase subunit D 2 (nuoD2) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00333, NADH dehydrogenase I subunit D [EC: 1.6.5.3] (inferred from 100% identity to smk:Sinme_6026)

MetaCyc: 48% identical to NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (Homo sapiens)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• NADH to cytochrome bd oxidase electron transfer I (1/2 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)
• Fe(II) oxidation (2/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

See P56908 at UniProt or InterPro

Protein Sequence (404 amino acids)

>SMa1529 NuoD2 NADH I chain D (Sinorhizobium meliloti 1021)
MTEVTELMRPEGEALNTKEVLLNLGPQHPSTHGVLRLVLQLDGEYVERVDPHIGYLHRGT
EKLAESFTYTQIFPLTDRLDYLCPPSNNLAFALAVEKLLGIEAPIRAQYIRVMMAELARI
SGHLLITGALPMDLGAMTALLYAMREREMIMDLLEMITGARMHTSYCRVGGVREDLPDGF
LPKIREFCEIFPNRIRDYERLIENNRVFLSRTQGVGVISATDAIDLGLSGPNLRASGVDW
DIRRDEPYEIYDRLDFDVITREEGDCYSRWLCRVDEMRESIRLIEQCMEQMPEGPFQVDI
PTIAFPVDKERVHCSMEALIQHFDLSAYGFDVPAGEVYSVIEAPKGELGFYIISDGSPKP
FRMKVRAPSFVNLQALFGVTNARYLADMIAVLGSLDPVMAEVDK