Protein Info for SMa1488 in Sinorhizobium meliloti 1021

Annotation: oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 747 signal peptide" amino acids 1 to 49 (49 residues), see Phobius details PF20256: MoCoBD_2" amino acids 61 to 194 (134 residues), 46.6 bits, see alignment E=3.5e-16 amino acids 607 to 692 (86 residues), 50.7 bits, see alignment E=1.9e-17 PF02738: MoCoBD_1" amino acids 334 to 566 (233 residues), 109.7 bits, see alignment E=1.3e-35

Best Hits

KEGG orthology group: K07303, isoquinoline 1-oxidoreductase, beta subunit [EC: 1.3.99.16] (inferred from 100% identity to smk:Sinme_6005)

MetaCyc: 64% identical to 2-deoxy-D-ribose dehydrogenase beta subunit (Pseudomonas simiae)
1.1.2.M2 [EC: 1.1.2.M2]

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.1.2.M2 or 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92YQ3 at UniProt or InterPro

Protein Sequence (747 amino acids)

>SMa1488 oxidoreductase (Sinorhizobium meliloti 1021)
MNDMTPVTSEITASEIGKPFKLPRRGFLGASLGALVLGVTLPAGRARAQAAAAAITPGTR
ISAFLEILPNETVLFRSAFIEGGQGIFTAMAQIVGEELDVDPMQFVVEGAPPGPDYLLTG
GGRFTGGSMSVRMSYDAMRKLGASARHMLIQAAAVRLRVPVSELSTEPGRVVHGASGRTL
PYGEIADAAAGLPLPTNVVLRDRADFRWIGKPVARLDVRDKSTGKARYAIDLKVDRMLHA
AVQHSPRLGGEPGALQNEADVRGMPGVHSIHSLPGTVAVVADSWWRARMAAEALQVTWTE
PTRGTAHVMPADFSTEAHMAMLKATPGEGVAYETVGNAATALGDAARVVEATYDAPYLVH
GQLEPPSALARWNDDGSLDLWVPNQAPEMFQAEAAKVAGIAPEKVTIHSPMLGGFFGRHF
LYQTANPFPQAILLAKAVARPVKLIWSREEEFLRDTLRPMGAVRFRAGLDAEGLPVALEA
VAVGEGPTGRWFGRQPDKVDSSSVEGIAGKVYAIPNRHIGQVHVDDPAIIGFWRSVGHSM
NDFFYETFFDEMADAGQQDPYELRRRLLADSPRHRTLLEAVAELSGGWRRGPFIADDGTR
RARGVAMASPFGSEVATIAEVSLRSGEVVVHDVWVAIDPGSIVNPAIIEAQVNSAVALGL
SSALLEEVVYVDGMPQARNYDGYPILTPDRMPRVHVRIVESGAPMGGIGEPGLPGVPPAI
ANAVSVLAGRRVRSLPLSKHDFKGVDG