Protein Info for SMa1306 in Sinorhizobium meliloti 1021

Annotation: VirB9 type IV secretion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR02781: P-type conjugative transfer protein VirB9" amino acids 5 to 256 (252 residues), 259.4 bits, see alignment E=1.8e-81 PF03524: CagX" amino acids 31 to 254 (224 residues), 184.3 bits, see alignment E=1.2e-58

Best Hits

Swiss-Prot: 61% identical to VIRB9_BARQU: Type IV secretion system protein virB9 (virB9) from Bartonella quintana (strain Toulouse)

KEGG orthology group: K03204, type IV secretion system protein VirB9 (inferred from 100% identity to sme:SMa1306)

Predicted SEED Role

"Outer membrane and periplasm component of type IV secretion of T-DNA complex, has secretin-like domain, VirB9"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92Z01 at UniProt or InterPro

Protein Sequence (284 amino acids)

>SMa1306 VirB9 type IV secretion protein (Sinorhizobium meliloti 1021)
MRTTFIATLLLTAAAPTALALEIPRGATQDSRVRFVDYQPYNITRIIGSLRSSVQVEFAP
DEEIAHVALGNSVAWEVAPAGNILFLKPRENQPVTNISVVTTRRDGSTRSYQMELTVRDG
KVEVGQNTYFYVKYRYPADEAERRRQAAAARAIAAQAKEADNVLAIHEAYGPRNWRYSAQ
GAQALEPQSVYDNGKVTTFAFVGNQEMPAIYIENSDGSESLVPKSVDGNLVLVHAISRKF
ILRRGGDVLCVFNEAYDRVGINPDTSTTSPSVERIVRIDAGAVQ