Protein Info for SMa1250 in Sinorhizobium meliloti 1021

Annotation: NirK nitrite reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 376 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details TIGR02376: nitrite reductase, copper-containing" amino acids 49 to 373 (325 residues), 552.7 bits, see alignment E=1.5e-170 PF07732: Cu-oxidase_3" amino acids 90 to 196 (107 residues), 35.8 bits, see alignment E=1.2e-12 PF07731: Cu-oxidase_2" amino acids 149 to 194 (46 residues), 24.2 bits, see alignment 3.8e-09 PF00394: Cu-oxidase" amino acids 211 to 360 (150 residues), 61.3 bits, see alignment E=1.9e-20

Best Hits

Swiss-Prot: 100% identical to NIRK_RHIME: Copper-containing nitrite reductase (nirK) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00368, nitrite reductase (NO-forming) [EC: 1.7.2.1] (inferred from 100% identity to sme:SMa1250)

Predicted SEED Role

"Copper-containing nitrite reductase (EC 1.7.2.1)" in subsystem Denitrification (EC 1.7.2.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92Z29 at UniProt or InterPro

Protein Sequence (376 amino acids)

>SMa1250 NirK nitrite reductase (Sinorhizobium meliloti 1021)
MSEQFQMTRRSMLAGAAIAGAVTPLIGAVSAHAEEAVAKTAHINVASLPRVKVDLVKPPF
VHAHTQKAEGGPKVVEFTLTIEEKKIVIDEQGTELHAMTFNGSVPGPLMVVHQDDYVELT
LINPDTNTLQHNIDFHSATGALGGGALTVVNPGDTTVLRFKASKAGVFVYHCAPPGMVPW
HVTSGMNGAIMVLPREGLTDGKGNSITYDKVYYVGEQDFYVPRDANGKFKKYESVGEAYA
DTLEVMRTLTPSHIVFNGAVGALTGDSALKAAVGEKVLIVHSQANRDTRPHLIGGHGDYV
WATGKFRNAPDVDQETWFIPGGTAGAAFYTFEQPGIYAYVNHNLIEAFELGAAAHFAVTG
DWNDDLMTSVRAPSGT