Protein Info for SMa1236 in Sinorhizobium meliloti 1021

Annotation: nitrate reductase catalytic subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 834 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details TIGR01706: periplasmic nitrate reductase, large subunit" amino acids 5 to 833 (829 residues), 1581 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 44 to 96 (53 residues), 63.2 bits, see alignment 2.7e-21 PF00384: Molybdopterin" amino acids 100 to 573 (474 residues), 253.6 bits, see alignment E=4.5e-79 PF01568: Molydop_binding" amino acids 720 to 827 (108 residues), 83.5 bits, see alignment E=1.6e-27

Best Hits

Swiss-Prot: 88% identical to NAPA_AGRFC: Periplasmic nitrate reductase (napA) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 88% identity to atu:Atu4408)

MetaCyc: 66% identical to periplasmic nitrate reductase subunit NapA (Escherichia coli K-12 substr. MG1655)
Nitrate reductase (cytochrome). [EC: 1.9.6.1]

Predicted SEED Role

"Periplasmic nitrate reductase precursor (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4 or 1.9.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92Z36 at UniProt or InterPro

Protein Sequence (834 amino acids)

>SMa1236 nitrate reductase catalytic subunit (Sinorhizobium meliloti 1021)
MTGELTRREMLKAHAAGIAAATAGIALPAAAQPVPGGVEALQITWSKAPCRFCGTGCGVM
VGVKEGQVVATHGDMQAEVNRGLNCIKGYFLSKIMYGTDRLKTPLLRKRNGAFAKDGEFE
PVSWDEAFDVMAEQAKKVLKDKGPTAVGMFGSGQWTIFEGYAATKLMRAGFRSNNLDPNA
RHCMASAAYAFMRTFGMDEPMGCYDDFEHADAFVLWGSNMAEMHPILWTRLADRRLGHEH
VKVAVLSTFTHRSMDLADIPIVFKPGTDLAILNYIANHIIQTGRVNEDFVRKHTTFMVGA
TDIGYGLRPDNPLEVKAVNAKDAAKMTPSDFESFKSFVSEYTLDKVVELTGVEAGFLEQL
ADLYADPKRKVMSLWTMGFNQHVRGVWVNQMVYNLHLLTGKISEPGNSPFSLTGQPSACG
TAREVGTFAHRLPADMTVTNPEHRKHAEEIWRIPHGIIPEKPGYHAVEQDRMLKDGKLNF
YWVQVNNNVQAAPNTQNETYQGYRNPDNFIVVSDVYPTITAMSADLILPAAMWVEKEGAY
GNAERRTHVWHQLVDAPGEARSDLWQMVEFSKRFTTDEVWSTDILDANPGYRGKTLYDVL
FKNGNVDSFPASEINKEYANREAEAFGFYIQKGLFEEYASFGRGHGHDLAPYDRYHDERG
LRWPVVDGKETLWRYREGYDPYVKPGEGVKFYGRPDGKAVILAVPYEPPAESPDDEYNVW
LVTGRVLEHWHSGSMTMRVPELYKAFPGAVCFMNAGDARDRGINQGAEVRIVSRRGEIRA
RVETRGRNRMPPGVIFVPWFDASRLINKVTLDATDPISKQTDFKKCAVKIVSVA