Protein Info for SMa1229 in Sinorhizobium meliloti 1021

Annotation: FixL oxygen regulated histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 505 transmembrane" amino acids 23 to 43 (21 residues), see Phobius details amino acids 49 to 68 (20 residues), see Phobius details amino acids 73 to 90 (18 residues), see Phobius details amino acids 98 to 118 (21 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 133 to 260 (128 residues), 117.5 bits, see alignment E=2.1e-38 PF00989: PAS" amino acids 138 to 249 (112 residues), 79.3 bits, see alignment E=8.1e-26 PF13188: PAS_8" amino acids 138 to 190 (53 residues), 25.9 bits, see alignment 2.5e-09 PF08448: PAS_4" amino acids 143 to 254 (112 residues), 40 bits, see alignment E=1.4e-13 PF13426: PAS_9" amino acids 147 to 252 (106 residues), 46.9 bits, see alignment E=1e-15 PF00512: HisKA" amino acids 276 to 342 (67 residues), 35.8 bits, see alignment E=2.3e-12 PF02518: HATPase_c" amino acids 389 to 495 (107 residues), 90.8 bits, see alignment E=2.8e-29 PF14501: HATPase_c_5" amino acids 391 to 483 (93 residues), 22.2 bits, see alignment E=3.8e-08

Best Hits

Swiss-Prot: 100% identical to FIXL_RHIME: Sensor protein FixL (fixL) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K14986, two-component system, LuxR family, sensor kinase FixL [EC: 2.7.13.3] (inferred from 100% identity to smk:Sinme_5887)

Predicted SEED Role

"Two-component oxygen-sensor histidine kinase FixL" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P10955 at UniProt or InterPro

Protein Sequence (505 amino acids)

>SMa1229 FixL oxygen regulated histidine kinase (Sinorhizobium meliloti 1021)
MLSKSGIERTQWGRRVVRWRGDGVAAYIVAAIVTSSVLAIRMIRAEPIGEGLLLFSFIPA
ILVVALIGGRNPILFAAGLSLVAAVSHQQISSADGPSVVELLVFGSAVLLIVALGEVLEA
ARRAIDRTEDVVRARDAHLRSILDTVPDATVVSATDGTIVSFNAAAVRQFGYAEEEVIGQ
NLRILMPEPYRHEHDGYLQRYMATGEKRIIGIDRVVSGQRKDGSTFPMKLAVGEMRSGGE
RFFTGFIRDLTEREESAARLEQIQAELARLARLNEMGEMASTLAHELNQPLSAIANYSHG
CTRLLRDMDDAVATRIREALEEVASQSLRAGQIIKHLREFVTKGETEKAPEDIRKLVEES
AALALVGSREQGVRTVFEYLPGAEMVLVDRIQVQQVLINLMRNAIEAMRHVDRRELTIRT
MPADPGEVAVVVEDTGGGIPEEVAGQLFKPFVTTKASGMGIGLSISKRIVEAHGGEMTVS
KNEAGGATFRFTLPAYLDERIVAND