Protein Info for SMa1209 in Sinorhizobium meliloti 1021

Annotation: FixI1 ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 757 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 42 to 59 (18 residues), see Phobius details amino acids 123 to 144 (22 residues), see Phobius details amino acids 155 to 176 (22 residues), see Phobius details amino acids 188 to 204 (17 residues), see Phobius details amino acids 216 to 234 (19 residues), see Phobius details amino acids 371 to 391 (21 residues), see Phobius details amino acids 397 to 425 (29 residues), see Phobius details amino acids 691 to 708 (18 residues), see Phobius details amino acids 714 to 732 (19 residues), see Phobius details TIGR01511: copper-translocating P-type ATPase" amino acids 171 to 735 (565 residues), 587.6 bits, see alignment E=5.7e-180 TIGR01525: heavy metal translocating P-type ATPase" amino acids 190 to 734 (545 residues), 548.2 bits, see alignment E=5.4e-168 TIGR01512: cadmium-translocating P-type ATPase" amino acids 220 to 735 (516 residues), 364.8 bits, see alignment E=1.4e-112 TIGR01494: HAD ATPase, P-type, family IC" amino acids 224 to 716 (493 residues), 223.4 bits, see alignment E=8.4e-70 PF00122: E1-E2_ATPase" amino acids 252 to 430 (179 residues), 163 bits, see alignment E=8.3e-52 PF00702: Hydrolase" amino acids 448 to 646 (199 residues), 88 bits, see alignment E=1.7e-28

Best Hits

Swiss-Prot: 100% identical to FIXI_RHIME: Nitrogen fixation protein FixI (fixI) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01552, [EC: 3.6.3.-] (inferred from 100% identity to sme:SMa1209)

Predicted SEED Role

"Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.4)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-, 3.6.3.4

Use Curated BLAST to search for 3.6.3.- or 3.6.3.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P18398 at UniProt or InterPro

Protein Sequence (757 amino acids)

>SMa1209 FixI1 ATPase (Sinorhizobium meliloti 1021)
MSCCASSAAIMVAEGGQASPASEELWLASRDLGGGLRQTELSVPNAYCGTCIATIEGALR
AKPEVERARVNLSSRRVSIVWKEEVGGRRTNPCDFLHAIAERGYQTHLFSPGEEEGDDLL
KQLILAVAVSGFAATNIMLLSVSVWSGADAATRDLFHWISALIAGPALIYAGRFFYKSAW
NAIRHGRTNMDVPIALAVSLSYGMSLHETIGHGEHAWFDASVTLLFFLLIGRTLDHMMRG
RARTAISGLARLSPRGATVVHPDGSREYRAVDEINPGDRLIVAAGERVPVDGRVLSGTSD
LDRSVVNGESSPTVVTTGDTVQAGTLNLTGPLTLEATAAARDSFIAEIIGLMEAAEGGRA
RYRRIADRAARYYSPAVHLLALLTFVGWMLVEGDVRHAMLVAVAVLIITCPCALGLAVPV
VQVVAAGRLFQGGVMVKDGSAMERLAEIDTVLLDKTGTLTIGKPRLVNAHEISPGRLATA
AAIAVHSRHPIAVAIQNSAGAASPIAGDIREIPGAGIEVKTEDGVYRLGSRDFAVGGSGP
DGRQSEAILSLDFRELACFRFEDQPRPASRESIEALGRLGIATGILSGDRAPVVAALASS
LGISNWYAELSPREKVQVCAAAAEAGHKALVVGDGINDAPVLRAAHVSMAPATAADVGRQ
AADFVFMHERLSAVPFAIETSRHAGQLIRQNFALAIGYNVIAVPIAILGYATPLVAAVAM
SSSSLVVVFNALRLKRSLAAGRGATPGTLIHSGAVTS