Protein Info for SMa1191 in Sinorhizobium meliloti 1021

Annotation: nitric oxide dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 transmembrane" amino acids 269 to 278 (10 residues), see Phobius details PF00042: Globin" amino acids 27 to 132 (106 residues), 63.7 bits, see alignment E=3.5e-21 PF00970: FAD_binding_6" amino acids 159 to 260 (102 residues), 25.4 bits, see alignment E=2.2e-09 PF00175: NAD_binding_1" amino acids 271 to 378 (108 residues), 71.1 bits, see alignment E=1.8e-23

Best Hits

Swiss-Prot: 100% identical to HMP_RHIME: Flavohemoprotein (hmp) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K05916, nitric oxide dioxygenase [EC: 1.14.12.17] (inferred from 100% identity to sme:SMa1191)

Predicted SEED Role

"Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)" in subsystem Bacterial hemoglobins or Flavohaemoglobin or Glutaredoxins (EC 1.14.12.17)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.12.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7WUM8 at UniProt or InterPro

Protein Sequence (403 amino acids)

>SMa1191 nitric oxide dioxygenase (Sinorhizobium meliloti 1021)
MLTQKTKDIVKATAPVLAQHGYAIIQHFYKRMFQAHPELKNIFNMAHQERGEQQQALARA
VYAYAANIENPESLSAVLKDIAHKHASLGVRPEQYPIVGEHLLASIKEVLGDAATDEIIS
AWAQAYGNLADILAGMESELYERSEERAGGWAGWRRFIVREKNPESDVITSFVLEPADGG
PVADFEPGQYTSVAVQVPKLGYQQIRQYSLSDSPNGRSYRISVKREDGGLGTPGYVSSLL
HDEINVGDELKLAAPYGNFYIDVSATTPIVLISGGVGLTPMVSMLKKALQTPPRKVVFVH
GARNSAVHAMRDRLKEASRTYPDFKLFIFYDEPLPTDIEGRDYDFAGLVDVEKVKDSILL
DDADYYICGPVPFMRMQHDKLLGLGITEARIHYEVFGPDLFAE