Protein Info for SMa1182 in Sinorhizobium meliloti 1021

Annotation: nitrous-oxide reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 639 signal peptide" amino acids 1 to 47 (47 residues), see Phobius details TIGR04244: nitrous-oxide reductase, TAT-dependent" amino acids 10 to 635 (626 residues), 1127.9 bits, see alignment E=0 PF18793: nos_propeller_2" amino acids 178 to 247 (70 residues), 125.9 bits, see alignment E=1.3e-40 PF18764: nos_propeller" amino acids 423 to 493 (71 residues), 119.7 bits, see alignment E=8.1e-39 PF13473: Cupredoxin_1" amino acids 547 to 634 (88 residues), 30.2 bits, see alignment E=8e-11 PF00116: COX2" amino acids 576 to 628 (53 residues), 23.5 bits, see alignment 9.1e-09

Best Hits

Swiss-Prot: 100% identical to NOSZ_RHIME: Nitrous-oxide reductase (nosZ) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00376, nitrous-oxide reductase [EC: 1.7.2.4] (inferred from 100% identity to sme:SMa1182)

Predicted SEED Role

"Nitrous-oxide reductase (EC 1.7.99.6)" in subsystem Denitrification (EC 1.7.99.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.2.4 or 1.7.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q59746 at UniProt or InterPro

Protein Sequence (639 amino acids)

>SMa1182 nitrous-oxide reductase (Sinorhizobium meliloti 1021)
MSNEETKMRLNRRQMLGTTAFMAAAGAVGAGGALTLSGGTATPARAQETSGSSYEVKPGE
LDEYYVFFSSGQSGEIRIVGAPSMREMMRIPVFNRCSATGWGQTNESRKVMTEGLLPETV
EFLKDQGGLYLNGDLHHPHPSFTDGTYDGRYLYANDKSNSRVCRIRLDVMKCDKIIQLPN
QHTVHGLRVQKYPKTGYVFCNGEDAVPVPNDGKTMGDKNSYQAIFTAVDGETMEVAWQVM
VDGNLDNVDADYQGKYCFATCYNSEEGFTLADMMASEQDWVVIFNLKRIEEAVAKGDYKE
IGGVPVLDGRKGSPYTRYVPVPNSPHGINTAPDGIHVVANGKLSPTVTVFDVRKFDDLFD
DKIQARDTVVAEPELGLGPLHTAYDGKGNAYTTLFIDSQVCKWNIEDAKRAYAGEKVDPI
RHKLDVHYQPGHNHTSMGQTKEADGKWLISLNKFSKDRYLNVGPLKPENDQLIDISGDEM
VLVHDNPTFAEPHDATIVHASKINPVHVWNRDDPFFADAVAQAKADNIDLMVDSEVIRDG
NKVRVYMTSAAPAFGLDDFTVKQGDEVTVYVTNIDEVEDLTHGFCIVNYGINMEVAPQAT
ASVTFKASRPGVYWYYCTWFCHAMHMEMKGRMLVEAQGA