Protein Info for SMa0892 in Sinorhizobium meliloti 1021

Annotation: DnaE3 DNA polymerase III alpha chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1087 TIGR00594: DNA polymerase III, alpha subunit" amino acids 4 to 982 (979 residues), 557.7 bits, see alignment E=2.9e-171 PF02811: PHP" amino acids 6 to 129 (124 residues), 38.8 bits, see alignment E=3e-13 PF07733: DNA_pol3_alpha" amino acids 272 to 525 (254 residues), 298.7 bits, see alignment E=1.1e-92 PF17657: DNA_pol3_finger" amino acids 528 to 694 (167 residues), 202.1 bits, see alignment E=9.5e-64 PF14579: HHH_6" amino acids 767 to 858 (92 residues), 68.8 bits, see alignment E=9.3e-23 PF01336: tRNA_anti-codon" amino acids 952 to 1020 (69 residues), 31.8 bits, see alignment 2.8e-11

Best Hits

Swiss-Prot: 100% identical to DNE22_RHIME: Error-prone DNA polymerase 2 (dnaE2-2) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 82% identity to agr:AGROH133_14628)

Predicted SEED Role

"Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7)" (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92ZJ6 at UniProt or InterPro

Protein Sequence (1087 amino acids)

>SMa0892 DnaE3 DNA polymerase III alpha chain (Sinorhizobium meliloti 1021)
MRYAELQVTTHFSFLRAASSAEELFATARLMGIEALGVVDRNSLAGIVRALEASRATGLR
LVVGCRLDLQDGMSILVYPTDRAAYSRLTRLLTLGKGRGGKANCIIHFDDVALYAEGLIG
ILVPDLADEVCAVQLRKIAEVFGDRAYVSLCLRRRPNDQLQLHELTNLAVKHRVKTIVTN
DVLFHEHGRRQLQDVVTCIRTGMTIDDVGFERERHADRYLKPPEEMARLFPAYPEALART
MEIVERCRFSLEELVYQYPEEALILGMTAQQSLQHYTWEGVRARYPEGLPTHVEKTIRHE
LALIETMKYAPYFLTVFSIVRYARSQGILCQGRGSAANSAVCYVLGITSIDPETNDLLFE
RFVSQERDEPPDIDVDFEHERREEVIQWIYKTYGHDKAALCSTVTRYRAKGAIRDVGKAL
DLPEDLIRTLSSGIWSWSETVGERQVRELGLNPDDRRLTLTLRLAQQLMGAPRNLSQHPG
GFVLTHDRLDDLVPIEPATMADRQVIEWDKDDIEALKFLKVDVLALGMLTCMAKAFALIS
EHKHEDIDLATIPQEDPATYAMIRKADTLGTFQIESRAQMSMLPRMKPRTFYDLVIQVAI
VRPGPIQGDMVHPYLRRREGKEKVEYPTPELEAVLHKTLGVPLFQESAMRVAMVCAGFTG
GEADQLRKSMATFKFTGGVSRFKDKLVNGMIRNGYTKEFAEKTFSQLEGFGSYGFPESHA
ASFALIAYASNYIKCYFPDVFCAALLNSQPMGFYAPAQIVRDAREHGVEVRPICINRSRW
DCMLEPIDGSGGHAVRLGMRLVRGLATADAARIVAARADEPFTSVDDMWRRSGVPVASLV
ELAEADAFLPSLSLERRDALWAIKALRDEPLPLFTAAADREARAIAEQEEPEVELRQMTD
GQNVVEDYSHTGLTLREHPLRFMRDDLAKRRIVTCAQAMTAHDGQWLMAAGLVLVRQRPG
SAKGVMFITIEDETGIANIVVWPKLFERSRRVVLGASMMAINGRIQREGEVVHLVAQQLF
DLSADLSSLAERDGAFRPPTGRGDEFAHGSPGSADSRGKAPPGVRARDILVPDLHIDTLK
IKSRNFQ