Protein Info for SMa0853 in Sinorhizobium meliloti 1021

Annotation: 3-ketoacyl-ACP synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 402 PF00109: ketoacyl-synt" amino acids 3 to 239 (237 residues), 183.4 bits, see alignment E=9.4e-58 PF00108: Thiolase_N" amino acids 143 to 189 (47 residues), 27.3 bits, see alignment 3.6e-10 PF02801: Ketoacyl-synt_C" amino acids 247 to 359 (113 residues), 121.6 bits, see alignment E=2.9e-39

Best Hits

Swiss-Prot: 100% identical to NODE_RHIME: Nodulation protein E (nodE) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K14660, nodulation protein E [EC: 2.3.1.-] (inferred from 99% identity to smd:Smed_6194)

MetaCyc: 42% identical to beta-ketoacyl-acyl carrier protein synthase II (Bacillus subtilis subtilis 168)
Beta-ketoacyl-acyl-carrier-protein synthase II. [EC: 2.3.1.179, 2.3.1.41]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-, 2.3.1.179, 2.3.1.41

Use Curated BLAST to search for 2.3.1.- or 2.3.1.179 or 2.3.1.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P06230 at UniProt or InterPro

Protein Sequence (402 amino acids)

>SMa0853 3-ketoacyl-ACP synthase (Sinorhizobium meliloti 1021)
MDRRVVITGMGGLCGLGTDTTSIWKWMREGRSAIGPLLNTELHGLKGIVGAEVKALPDHN
IDRKQLVSMDRISVLAVIAAHEAMRQAGLSCNEGNALRFGATVGVGLGGWDATEKAYRTL
LVDGGTRTEIFTGVKAMPSAAACQVSMSLGLRGPVFGVTSACSSANHAIASAVDQIKCGR
ADVMLAGGSDAPLVWIVLKAWEAMRALAPDTCRPFSAGRKGVVLGEGAGMAVLESYEHAT
ARGATILAEVAGVGLSADAFHITAPAVHGPESAMRACLADAGLNAEDVDYLNAHGTGTKA
NDQNETTAIKRVFGDHAYSMSISSTKSTHAHCIGAASALEMIACVMAIQEGVVPPTANYR
EPDPDCDLDVTPNVPRERKVRVAMSNAFAMGGTNAVLAFKQV