Protein Info for SMa0817 in Sinorhizobium meliloti 1021

Annotation: oxidoreductase FixC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF00890: FAD_binding_2" amino acids 7 to 42 (36 residues), 22.6 bits, see alignment 1.5e-08 PF12831: FAD_oxidored" amino acids 7 to 162 (156 residues), 29 bits, see alignment E=1.8e-10 PF13450: NAD_binding_8" amino acids 10 to 41 (32 residues), 26.5 bits, see alignment (E = 1.5e-09)

Best Hits

Swiss-Prot: 100% identical to FIXC_RHIME: Protein FixC (fixC) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00313, electron transfer flavoprotein-quinone oxidoreductase [EC: 1.5.5.-] (inferred from 100% identity to smk:Sinme_6356)

Predicted SEED Role

"Probable electron transfer flavoprotein-quinone oxidoreductase FixC (EC 1.5.5.-)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 1.5.5.-)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.5.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P09820 at UniProt or InterPro

Protein Sequence (435 amino acids)

>SMa0817 oxidoreductase FixC (Sinorhizobium meliloti 1021)
MTKEKFDAIVVGAGMSGNAAAYAMASRGLKVLQLERGEYPGSKNVQGAIMYANMLEAIIP
DFRNDAPLERHLVEQRFWIMDDTSHTGMHYRSDDFNEVTPNRYTIIRAQFDKWLSRKVCE
AGGTVLCETTATGLEWDSAGKAIGVRTDRAGDVVLADVVVLAEGVNGLLGTRAGLREMPK
SKNVALAVKELHFLPEEVIAERFGLTGDEGCVIEAGGTISRGMAGLGFLYTNKESISLGI
GCLISNFAETMERPYALLDAFKRHPSIQPLIAGSEVKEYAAHLIPEGGFNAIPRLCGNGW
VVVGDAAQLNNAVHREGSNLAMASGRMAGEAISIIKSRGGVMDKASLSLYKTMLDKSFVV
EDLSKQKDMPSLLHTNSPNFFTTYPQLISHAAQNFVRVDGTPKIEREIATTAAFLRARSR
WGLVSDAVRLASAWR