Protein Info for SMa0816 in Sinorhizobium meliloti 1021

Annotation: FixX ferredoxin-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 25 50 75 98 PF05187: Fer4_ETF_QO" amino acids 43 to 92 (50 residues), 28.3 bits, see alignment E=8.4e-11

Best Hits

Swiss-Prot: 100% identical to FIXX_RHIME: Ferredoxin-like protein (fixX) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K03855, ferredoxin like protein (inferred from 98% identity to smd:Smed_6231)

MetaCyc: 52% identical to quinone reductase (NADH,flavodoxin) complex iron-sulfur prrotein subunit (Azotobacter vinelandii)
1.19.1.M1 [EC: 1.19.1.M1]

Predicted SEED Role

"Ferredoxin-like protein" in subsystem Acetyl-CoA fermentation to Butyrate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.19.1.M1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P09822 at UniProt or InterPro

Protein Sequence (98 amino acids)

>SMa0816 FixX ferredoxin-like protein (Sinorhizobium meliloti 1021)
MKTAIAERIEDKLYQNRYLVDAGRPHITVRPHRSPSLNLLALTRVCPAKCYELNETGQVE
VTADGCMECGTCRVLCEANGDVEWSYPRGGFGVLFKFG