Protein Info for SMa0815 in Sinorhizobium meliloti 1021

Annotation: Fis family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 541 TIGR01817: Nif-specific regulatory protein" amino acids 6 to 541 (536 residues), 811.5 bits, see alignment E=1.9e-248 PF01590: GAF" amino acids 24 to 158 (135 residues), 29 bits, see alignment E=3.5e-10 PF00158: Sigma54_activat" amino acids 200 to 366 (167 residues), 235.6 bits, see alignment E=6.7e-74 PF14532: Sigma54_activ_2" amino acids 201 to 371 (171 residues), 70.5 bits, see alignment E=4.4e-23 PF07728: AAA_5" amino acids 224 to 342 (119 residues), 30.7 bits, see alignment E=7.4e-11 PF02954: HTH_8" amino acids 497 to 533 (37 residues), 33.9 bits, see alignment 5.3e-12

Best Hits

Swiss-Prot: 100% identical to NIFA_RHIME: Nif-specific regulatory protein (nifA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K02584, Nif-specific regulatory protein (inferred from 100% identity to sme:SMa0815)

Predicted SEED Role

"Nitrogenase (molybdenum-iron)-specific transcriptional regulator NifA" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P03028 at UniProt or InterPro

Protein Sequence (541 amino acids)

>SMa0815 Fis family transcriptional regulator (Sinorhizobium meliloti 1021)
MRKQDKRSAEIYSISKALMAPTRLETTLNNFVNTLSLILRMRRGGLEIPASEGETKITAA
TRNSGSPSAADYTVPKAAIDQVMTAGRLVVPDVCNSELFKDQIKWRGIGPTAFIAAAVEV
DHETGGMLWFECAEESDYDYEEEVHFLSMAANLAGRAIRLHRTISRRERTFAEEQQEQQN
SRDEQSQSSARQRLLKNDGIIGESTALMTAVDTAKVMAETNSIVLLRGETGTGKECFAKL
IHQHSTRQKKPFIKFNCPALSESLLESELFGHEKGAFTGAIAQRVGRFESANGGTLLLDE
IGEIPPAFQAKLLRVIQEGEFERVGGTKTLKVDVRLIFATNKDLEMAVQNGEFREDLYYR
ISGVPLILPPLRHRDGDIPLLARAFLQRFNEENGRDLHFAPSALDHLSKCKFPGNVRELE
NCVRRTATLARSKTITSSDFACQTDQCFSSRLWKGVHCSHGHIEIDAPAGTTPLLGAPAN
DVPPKEPGSAGVASNLIERDRLISALEEAGWNQAKAARILEKTPRQVGYALRRHGVDVRK
L