Protein Info for SMa0464 in Sinorhizobium meliloti 1021

Annotation: guanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1058 PF00211: Guanylate_cyc" amino acids 31 to 191 (161 residues), 55.2 bits, see alignment E=1e-18 PF13191: AAA_16" amino acids 223 to 392 (170 residues), 81 bits, see alignment E=2.2e-26 PF13401: AAA_22" amino acids 246 to 404 (159 residues), 28 bits, see alignment E=3.7e-10

Best Hits

KEGG orthology group: None (inferred from 100% identity to sme:SMa0464)

Predicted SEED Role

"Adenylate cyclase (EC 4.6.1.1) / Guanylate cyclase (EC 4.6.1.2)" in subsystem cAMP signaling in bacteria (EC 4.6.1.1, EC 4.6.1.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.6.1.1, 4.6.1.2

Use Curated BLAST to search for 4.6.1.1 or 4.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q930F6 at UniProt or InterPro

Protein Sequence (1058 amino acids)

>SMa0464 guanylate cyclase (Sinorhizobium meliloti 1021)
MAVIRRPSRSLTPTEEIALARPEPPAEDVRKTITILIADIVDSSRLSLTLDPEALRELFA
RYFDEMTSTIQRHGGVVDRYVGDEILAVFGVPTLHEDDALRAVSAAVDMRDTLARLNHEF
ETGWGVQLAHRIGLNTGEVFTGIDRWGHRFLTGEAVRVAKRLQEAAAANEILMGEATHKL
VRHAVVVESSSPRAVKHGETFPAIIVLTVIARTTGFQRRFDTPFVGRKRQLAMISTLLGD
FVSNRTCHLLTVLGEAGVGKSRLVSEVAGNLAREMTVAHGRCLPYGDGITYWLLADIVRE
IFRAGGGDSGKLSVAAIAEVLAGVDKAKLIAERIAGLLGFGAGDPGTREETFWAVRRLFE
VFARERPVVIVVEDLHWADPTLLDFIEHLVDFSHGFPIMIVATARPELLDTRPGWGGGTP
NATTIALEPLSEAESRDMVLNLLHRLPLSPAVELMITRAVGGNPLFAEELVAMLVDEELL
RRNEDCWVAREDLSELPVPSTIIALLAARLEGLTSQERAILTAAAVEGAVFHRSAVDELA
RPAPKALGDGLLSLVRRDLIRPEAPSFVGEETYRFRHDMIREAAYRSLPKNARADLHERF
ASWLEITAKERLREFEEVVGYHLEQAFQYRIALGPRGARAASLAARACERLEAAGRRALV
RSDLSAAISLLERVSRLLLADDPRRIALLAELSGALIESGRLDDAGRVLEEAGGLAGAAM
DRRLAAHVRVQRQFLRLLHGEEGGLEKAAQAAAEVIPVFEGFGDDLGLCRARRLEAWLFF
NGARGEAAAAAWERAAAHARRAGNLHEYYEILTWIASSLWFGPTPAAEGIRRCEAMRAEV
GESLESEAAILRQLACLNAVVGRFAIARELIAASNATYADLGLTLYVASSEHEAVVELLA
GNPAAAERSARAAYRALEEMGERAFRSTMAASLAVVILEQGRDEEAEDFAKLSAQLAASG
DLVTQVRWRRVRARVLARRAEISAAEALAREALEIAEKTDFINDRADALVDLSHVLEASR
RRDEAVAAATGAVHLYELKGNVVAAAATRLRLGKLVAM