Protein Info for SMa0233 in Sinorhizobium meliloti 1021

Annotation: OtsA trehalose-6-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 TIGR02400: alpha,alpha-trehalose-phosphate synthase (UDP-forming)" amino acids 3 to 456 (454 residues), 618.4 bits, see alignment E=3.6e-190 PF00982: Glyco_transf_20" amino acids 22 to 456 (435 residues), 406.7 bits, see alignment E=6.6e-126

Best Hits

Swiss-Prot: 92% identical to OTSA_SINFN: Trehalose-6-phosphate synthase (otsA) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K00697, alpha,alpha-trehalose-phosphate synthase (UDP-forming) [EC: 2.4.1.15] (inferred from 100% identity to smk:Sinme_5369)

Predicted SEED Role

"Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15)" in subsystem Trehalose Biosynthesis (EC 2.4.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q930S0 at UniProt or InterPro

Protein Sequence (463 amino acids)

>SMa0233 OtsA trehalose-6-phosphate synthase (Sinorhizobium meliloti 1021)
MSRLVIVSNRVPVPDKGGIAPAGGLAVALKVALEEHGGIWMGWSGRSSGENEPEPLAQLH
QGNITYALTDLTDTDVGEYYHGFANRVLWPICHYRLDLAEYGRKEMAGYFRVNRFFAHRL
APLVRPDDVIWVHDYHLIPLAAELRQMGLKNRIGFFLHIPWPPADVLFTMPVHEEIMRGL
SHYDVVGFQTDHDLENFAGCLRREGIGDELGGGRFSAYGRVFKGGIYAIGIETAAFAEFA
KKALTNKTVRKARESIEHRSLIIGVDRLDYSKGITQRIDAFERFILANPAQQGRVTYLQI
TPKSRSEVPEYEAMQRTVAEQAGRVNGALGAVDWVPIRYINRSVGRHILAGLYRLGKVGL
VTPLRDGMNLVAKEYVAAQDPDDPGVLVLSRFAGAARDLKGALLVNPYDIEGTANAMARA
LSMPLEERKDRWKTMMDHLLEHDVSRWCRDFLNDLATSPDPSG