Protein Info for SMa0137 in Sinorhizobium meliloti 1021

Annotation: Diguanylate cyclase/phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 733 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 265 to 286 (22 residues), see Phobius details PF05228: CHASE4" amino acids 54 to 223 (170 residues), 86.3 bits, see alignment E=3.3e-28 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 300 to 461 (162 residues), 120 bits, see alignment E=4.4e-39 PF00990: GGDEF" amino acids 304 to 458 (155 residues), 134.1 bits, see alignment E=5.9e-43 PF00563: EAL" amino acids 478 to 709 (232 residues), 227.7 bits, see alignment E=2.1e-71

Best Hits

KEGG orthology group: None (inferred from 100% identity to smk:Sinme_5427)

Predicted SEED Role

"FIG01075258: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q930X2 at UniProt or InterPro

Protein Sequence (733 amino acids)

>SMa0137 Diguanylate cyclase/phosphodiesterase (Sinorhizobium meliloti 1021)
MRFVAGKVAVFRWMLPLAIAMALMGGIYLAVRWSAQQSDAVAVKRQQHLVELVISKMQGS
IAHDQESVTVWDDAVRKVSQEWDPRWVDSNLGSWMNSYFRHDGAFVISPDRKPLYAFLAG
QTNEQEAFSEIGPEAMPLIAKLQERLAAGDEGGTSEQVLSIGESDLVRIGGRPAIISVKP
IVSDTGDIVQEPGREYLHLAARFLDGDFLTHLGDDYGFEDLRFSVLPELGRQRSYAPIVS
SSRETIGYFSWLPFRPGADVMKATAPVLLVAGALLFALTSALSVVLRRRSRRLQESQAEL
DHLARHDPLTGLANRASFNRLLARVVATSTIDQANALLYLDLDRFKQVNDTLGHPVGDRL
MVEVAKRLKETAAGAAISRIGGDEFTIIVARTRQDEVEKLCDALIAAIRRPFEIDGQPIL
IGLSIGVAVATGNDADPIEITRKADIALYHAKSAGRNRYAVFGPHMDELIRTKRDLEQDL
RAALDAGNQLEVFYQPVYSAQSHEISSLEALIRWRHPSKGPIAPDAFVPLAETAGLIDRL
GAFVLKEACSTAREFPGLDIAVNVSAVELAQRHYAAQVLSLLRQFGIEPSRLELEITETA
LLDEAGVCEKNITALREFGVKFALDDFGTGFSSFGRLQRLEVDRIKIDRSFVHAFDRPGG
GVAVVQSIVGIAHAKGLRITAEGVETEEQSEILRKLGCDELQGFHLSRPMSKNSLRQLLG
ADKAQGTSSSSRS