Protein Info for SMa0002 in Sinorhizobium meliloti 1021

Annotation: formate dehydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1034 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details PF10518: TAT_signal" amino acids 9 to 29 (21 residues), 24.1 bits, see alignment (E = 5.1e-09) TIGR01553: formate dehydrogenase-N alpha subunit" amino acids 10 to 1024 (1015 residues), 1398.7 bits, see alignment E=0 TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 10 to 36 (27 residues), 19.2 bits, see alignment (E = 1.2e-07) PF04879: Molybdop_Fe4S4" amino acids 53 to 112 (60 residues), 52.2 bits, see alignment 9.6e-18 PF00384: Molybdopterin" amino acids 116 to 602 (487 residues), 97.3 bits, see alignment E=2e-31 PF01568: Molydop_binding" amino acids 903 to 1018 (116 residues), 55.5 bits, see alignment E=1.1e-18

Best Hits

Swiss-Prot: 61% identical to FDOG_ECOLI: Formate dehydrogenase-O major subunit (fdoG) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 62% identity to bpl:BURPS1106A_A2259)

MetaCyc: 61% identical to formate dehydrogenase O subunit alpha (Escherichia coli K-12 substr. MG1655)
FORMATEDEHYDROG-RXN [EC: 1.17.5.3]

Predicted SEED Role

"Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.5.3 or 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q931E0 at UniProt or InterPro

Protein Sequence (1034 amino acids)

>SMa0002 formate dehydrogenase subunit alpha (Sinorhizobium meliloti 1021)
MEAVPMNVDLSRRSFLKLAGAGAAATSLGAMGFGEAEAAVVAHVRPHKLTTTTETRNTCP
YCSVACGVIIYSKGDLRKGEAADIIHIEGDADHPTNRGTLCPKGAALKDFVKSPTRLQYP
MHRKPGSDKFERISWEDAFDRIARLMKDDRDANFIAANAAGVPVNRWTTVGMLAASATTN
ETAWATFKFAKALGIVGFDNQARVUHGPTVSSLGPTFGRGAMTNSWTDIKNTDLVVVMGG
NAAEAHPCGFKWVTEAKATRGAKLIVVDPRYTRTASVSDYYAPIRQGTDIAFLNGVMKYC
IDNDKVQWDYMKAFTNASYLVKDGFGYQDGLFTGYDAEKRDYDKSTWDYVLGDDGFVVTD
PALQHPRCVWNLLKAHLAPYTPEMVERICGTPKDKFLKVAEMISECSSPTKTMTSMYALG
WTQHSSGSQNIRAMAMLQLILGNIGVRGGGMNALRGHSNIQGLTDLGLMSHLLTGYLTMP
TEKDVDFTTYMSTRQFKPLRPGQTSYWQNYRKFMVSFQKAMWGDAARIDNDWAFNYLSKL
DVPAYDVLRVFELMYAGKVNGYICQGFNPLLAFPNRDKNTKALSNLKWLVTMDPLDTETA
RFWENHGDFNPVDTASIQTEVFQLPTTCFAEEEGSLTNSGRWLQWHWAGGTPPGEAKHDT
YIVAQIFLRMKEMYRNEGGAFPDPILNLSWDYADPNEPTPEELAKEINGRALTDLMDPAN
PMKVQVAAGKQILNFSQLRDDGSTMCGCWIYSGNFNEQGNNMARRDNHDPDDTGAYLGWS
FAWPLNRRTLYNRASADLQGKPWDPSRKLLEWDGTKWAGYDVPDIAPTAKPDEIGPFIMN
QEGTARLFSRGLMRDGPFPAHMEPFESPVANVFNPKMRGNPVSRVFQTDVAQMGLSDEFP
YAATSYRLTEHFHYWTKHNRVNSALQPEFFVEISEELAEEKNIENGGWVRVWSKRGSVKA
KAVVTKRIRPLMCDGKPVHVVGIPLHWGFTGSAKKGLGPNSLAPFVGDANIETPEYKAFL
VNIEPSTAPEEATV