Protein Info for SM_b21652 in Sinorhizobium meliloti 1021

Updated annotation (from data): ABC transporter for Lactose, periplasmic substrate-binding component
Rationale: Specific phenotype on Beta-Lactose. (KEGG_correct)
Original annotation: lactose ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF01547: SBP_bac_1" amino acids 44 to 328 (285 residues), 95.3 bits, see alignment E=7.1e-31 PF13416: SBP_bac_8" amino acids 51 to 330 (280 residues), 122.5 bits, see alignment E=3e-39

Best Hits

Swiss-Prot: 72% identical to LACE_RHIRD: Lactose-binding protein (lacE) from Rhizobium radiobacter

KEGG orthology group: K10188, lactose/L-arabinose transport system substrate-binding protein (inferred from 100% identity to smk:Sinme_4172)

Predicted SEED Role

"ABC transporter sugar binding protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q926I0 at UniProt or InterPro

Protein Sequence (424 amino acids)

>SM_b21652 ABC transporter for Lactose, periplasmic substrate-binding component (Sinorhizobium meliloti 1021)
MDIQTRAYLPRIAGLALAGASFLGVTAAQAKEITIWCWDPNFNVAIMKEAGARYTKTHPD
VTFNIVDFAKLDVEQKLQTGLSSGTADALPDIVLIEDYGAQKYLQSFPGAFAPLSGTVDY
SGFAPYKVELMTLDGQVYGMPFDSGVTGLYYRKDYLEAAGFKPEDMQDLTWDRFIEIGKQ
VEEKTGKKMMGLDPNDAGLVRIIMQSAGQWYFDKEGKPNIAGNAALKAALETIGKIMQAN
IYKPANGWSDWVGTFTSGDVATVVTGVWITGTVKAQPDQSGNWGVAPIPALSIEGATHAS
NLGGSSWYVLESSEEKAEAIDFLNEIYAKDIDFYQKILQDRGAVGSLLAARGGAAYEAAD
PFFGGEKVWQNFSDWLAKVPSVNYGIFTNEADLAVTAQLPAVTQGTPVDEVLQAIEAEVS
AQIQ