Protein Info for SM_b21407 in Sinorhizobium meliloti 1021

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 272 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details TIGR02772: Ku protein" amino acids 5 to 265 (261 residues), 298.4 bits, see alignment E=2.3e-93 PF02735: Ku" amino acids 14 to 177 (164 residues), 152.8 bits, see alignment E=5.4e-49

Best Hits

Swiss-Prot: 62% identical to KU_METSB: Non-homologous end joining protein Ku (ku) from Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)

KEGG orthology group: K10979, DNA end-binding protein Ku (inferred from 100% identity to smk:Sinme_4489)

Predicted SEED Role

"Ku domain protein" in subsystem DNA Repair Base Excision

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92UA2 at UniProt or InterPro

Protein Sequence (272 amino acids)

>SM_b21407 hypothetical protein (Sinorhizobium meliloti 1021)
MAPPRPYWKGYLKLSLVTCPVSMMPATSESEKVRFHTLNRKSNNRVVSRYVDAVTGKEVD
EDDEVKGYERGENDFLVIEDEELESVALESARTIDIEKFVPRDTIEWIWLEKPHYLTPSD
EVGHEAFSVIRDAMVAEKVAGISRLVIGRRERAVMLEPCGKGIVVWTLRYGDEVRKPENY
FADIGDGDNDPKLMSLVTSLIEERSKPWNPNMVSDPVQEKLLEIIESKRKSAKKVAKPKA
KEGEPVHAGNVIDLMAALKGSLEKKKPGGKKS