Protein Info for SM_b21166 in Sinorhizobium meliloti 1021

Updated annotation (from data): Imidazolonepropionase (EC 3.5.2.7)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (IMIDAZOLONEPROPIONASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: imidazolonepropionase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01224: imidazolonepropionase
  • amino acids 89 to 467 (379 residues), 439.3 bits, E=5.4e-136
• PF01979: Amidohydro_1
  • amino acids 127 to 444 (318 residues), 70.5 bits, E=1.6e-23
• PF07969: Amidohydro_3
  • amino acids 177 to 445 (269 residues), 63.8 bits, E=2.2e-21

Best Hits

Swiss-Prot: 100% identical to HUTI_RHIME: Imidazolonepropionase (hutI) from Rhizobium meliloti (strain 1021)

KEGG orthology group: None (inferred from 100% identity to sme:SM_b21166)

MetaCyc: 54% identical to imidazolonepropionase (Pseudomonas fluorescens)
Imidazolonepropionase. [EC: 3.5.2.7]

Predicted SEED Role

"Imidazolonepropionase (EC 3.5.2.7)" in subsystem Histidine Degradation (EC 3.5.2.7)

MetaCyc Pathways

• L-histidine degradation II (5/5 steps found)
• L-histidine degradation VI (7/8 steps found)
• L-histidine degradation I (4/4 steps found)
• L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.7

Sequence Analysis Tools

See O31196 at UniProt or InterPro

Protein Sequence (471 amino acids)

>SM_b21166 Imidazolonepropionase (EC 3.5.2.7) (Sinorhizobium meliloti 1021)
MPGGVAGARSQRPPPMPILFPCFWPAAPIALSHGSYFSLVIAGIMYIHYWRRRAAPMDGN
ENPNPARTSLWRNARLATLREELGPLGIIEDGVIAVRGERIVYAGPEAGLPSELARADQV
FDCEGRWVTPALIDCHTHIVHGGNRAREFQLRLEGATYEEIARAGGGIASTVEATNALSV
EALVEAALPRLDTLLAEGVSTVEVKSGYGLNVEAELKMLRAARRLESLRPVRIVTSYLAA
HATPPEFRGRNGDYIAEVVLPGLTAAHAEGLADAVDGFCEGIAFSPAEIASVFGRAKSLG
LPVKLHAEQLSDLGGAKLAASYGALSADHLEYLDAAGAAAMAKAGTVAVLLPGAFYTLRE
KQLPPVEALREAGTRIAIATDCNPGTSPLTSLLLTLNMSATLFRLTLEECLAGVTREAAR
ALGILGETGTIEAGKSADLAIWNIDQPAELIYRIGFNPLRERIFKGERILR