Protein Info for SM_b20984 in Sinorhizobium meliloti 1021

Updated annotation (from data): Nitrite reductase (NAD(P)H) (EC 1.7.1.4)
Rationale: Specifically important for utilizing Sodium nitrate. Automated validation from mutant phenotype: the predicted function (1.7.1.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: nitrite reductase [NAD(P)H], large subunit protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 820 PF07992: Pyr_redox_2" amino acids 8 to 287 (280 residues), 194.8 bits, see alignment E=8e-61 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 9 to 799 (791 residues), 903.8 bits, see alignment E=6e-276 PF13738: Pyr_redox_3" amino acids 89 to 258 (170 residues), 41.1 bits, see alignment E=4.9e-14 PF00070: Pyr_redox" amino acids 150 to 229 (80 residues), 66.7 bits, see alignment E=7.6e-22 PF18267: Rubredoxin_C" amino acids 321 to 387 (67 residues), 66.5 bits, see alignment E=6e-22 PF04324: Fer2_BFD" amino acids 424 to 471 (48 residues), 45.6 bits, see alignment 2.5e-15 amino acids 487 to 536 (50 residues), 44.3 bits, see alignment 6.3e-15 PF03460: NIR_SIR_ferr" amino acids 562 to 625 (64 residues), 52.7 bits, see alignment 1e-17 PF01077: NIR_SIR" amino acids 635 to 776 (142 residues), 116.9 bits, see alignment E=2.2e-37

Best Hits

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 100% identity to sme:SM_b20984)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92UL8 at UniProt or InterPro

Protein Sequence (820 amino acids)

>SM_b20984 Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (Sinorhizobium meliloti 1021)
MTETAREKLVIVGNGMAPGRMLEELFEKAPGRYAVTIFNAEPRVNYDRIMLSPVLSGEKE
YEEIIIHGDGWYIKHGITLYKGHKIIAIDRDAKTVTSDHGVTESYDKLVIATGSVPFIIP
VPGKELRGVITYRDLDDVQAMLLAAQSREKAVVIGGGLLGLEAAAGLQARGMDVTVLHVM
PTLMERQLDPAAGYLLQKAVEERGIKVVTKANTKRILGEEKVEGIELDDGRIIPATLVVM
AVGIRPNAGLAKEAGLAVNRGIVVDAGMQTSDGDIMALGECAEVGGMVYGLVAPLYEMAR
VAASHLAGDRRAAFAHSDTPTKLKVTGINLYSVGDFADADGREEIVLRDATAGIYKRLVL
KDNRIIGTVLYGDTADGAWFNDLLKRGTDISEMRDTLIFGQAYQGGSPLDPTAAVAALPD
DAEICGCNGVCKGKIVGAITSKGLTSLDDVRAHTKASASCGSCAGLVEQIMSLTLGDTYN
PASVQPMCNCTDLGHDDVRRLIKAKKLKSIPAVMQELEWKTSCGCAKCRPALNYYLVCDW
PDEYADDYQSRFINERVHANIQKDGTYSVVPRMWGGVTNSKELRAIADVVDKFNVPLVKV
TGGQRIDLLGIEKEDLPAVWADLGQAGFVSGQAYAKGLRTVKTCVGSDWCRFGTQDSTGL
GIRIEKFMWGSWTPAKLKMAVSGCPRNCAEATCKDVGVICVDSGFEIHFAGAAGLDIKGT
EVLGLVRTEDEALEHIVALTQMYREQARYLERIYKWAKRVGLDEIRRQIMDDVEKRKAYF
DRFVFSQKFAQVDPWSERVSGKDKHEFRPMATVGFNQAAE