Protein Info for SM_b20932 in Sinorhizobium meliloti 1021

Annotation: endo-1,3-1,4-beta-glycanase, C-terminal secretion signal protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 465 PF00353: HemolysinCabind" amino acids 31 to 59 (29 residues), 11.5 bits, see alignment (E = 3.8e-05) amino acids 105 to 139 (35 residues), 20.7 bits, see alignment 4.8e-08 amino acids 115 to 146 (32 residues), 19.6 bits, see alignment (E = 1.1e-07) PF00722: Glyco_hydro_16" amino acids 267 to 454 (188 residues), 98.4 bits, see alignment E=5.1e-32

Best Hits

Swiss-Prot: 100% identical to EXSH_RHIME: Endo-1,3-1,4-beta-glycanase ExsH (exsH) from Rhizobium meliloti (strain 1021)

KEGG orthology group: None (inferred from 100% identity to smk:Sinme_4657)

Predicted SEED Role

"endo-1,3-1,4-beta-glycanase, C-terminal secretion signal protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See O33680 at UniProt or InterPro

Protein Sequence (465 amino acids)

>SM_b20932 endo-1,3-1,4-beta-glycanase, C-terminal secretion signal protein (Sinorhizobium meliloti 1021)
MSKTVLNAVGTPLYYSGSSTAWFSATGSGPTLHGTAGNDSMWGDSSVNVTMIGGRGDDIY
YLYSSINRAYEAAGEGVDTISTWMSYTLPANFENLTVTGSGRFAFGNEADNIIKGGSGTQ
TIDGRGGNDVLIGAGGADTFVFARGNGSDLITDFNYDDIVRLDGYGFTSFEQILSNVAQE
GADLRLHLADGESLVFANTTADELQAHQFRLSLDRSVLSQTFSDEFNTLQLRNGTSGVWD
AKFWWAPEKGATLSSNGEQQWYINPSYEPTASVNPFSVNNGVLTITAAPASEAIQAEING
YDYTSGMLTTYSSFAQTYGYFEMRADMPDDQGVWPAFWLLPADGSWPPELDVVEMRGQDS
NTVIATVHSNETGSRTSIENSVKVADASGFHTYGVLWTEEEIVWYFDDAAIARADTPSDM
HDPMYMLVNLAVGGIAGTPRDGLADGSEMKIDYIKAYSLDADWQI