Protein Info for SM_b20612 in Sinorhizobium meliloti 1021

Annotation: C4-dicarboxylate transport sensor protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 621 signal peptide" amino acids 1 to 46 (46 residues), see Phobius details transmembrane" amino acids 319 to 338 (20 residues), see Phobius details PF00512: HisKA" amino acids 406 to 471 (66 residues), 33.8 bits, see alignment E=5.8e-12 PF02518: HATPase_c" amino acids 516 to 620 (105 residues), 85.8 bits, see alignment E=5.9e-28 PF14501: HATPase_c_5" amino acids 520 to 607 (88 residues), 25.8 bits, see alignment E=1.7e-09

Best Hits

Swiss-Prot: 100% identical to DCTB_RHIME: C4-dicarboxylate transport sensor protein DctB (dctB) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K10125, two-component system, NtrC family, C4-dicarboxylate transport sensor histidine kinase DctB [EC: 2.7.13.3] (inferred from 100% identity to sme:SM_b20612)

Predicted SEED Role

"Signal transduction histidine kinase regulating C4-dicarboxylate transport system"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P13633 at UniProt or InterPro

Protein Sequence (621 amino acids)

>SM_b20612 C4-dicarboxylate transport sensor protein (Sinorhizobium meliloti 1021)
MHHVRMVKLPAEASDPHALRSRARRSWLVFAAVALVLLAAGLLLARDYGRSQALAGLAGQ
SRIDASLKASLLRAVVERQRALPLVLADDAAIRGALLSPDRPSLDRINRKLEALATSAEA
AVIYLIDRSGVAVAASNWQEPTSFVGNDYAFRDYFRLAVRDGMAEHFAMGTVSNRPGLYI
SRRVDGPGGPLGVIVAKLEFDGVEADWQASGKPAYVTDRRGIVLITSLPSWRFMTTKPIA
EDRLAPIRESLQFGDAPLLPLPFRKIEARPDGSSTLDALLPGDSTAAFLRVETMVPSTNW
RLEQLSPLKAPLAAGAREAQLLTLAALVPLLALAALLLRRRQVVAMRSAEERLARNALEA
SVEERTRDLRMARDRLETEIADHRQTTEKLQAVQQDLVQANRLAILGQVAAGVAHEINQP
VATIRAYADNARTFLHRGQTVTAAENMESIAELTERVGAITDELRRFARKGHFAAGPTAM
KEVVEGALMLLRSRFAGRMDAIRLDLPPDGLQALGNRIRLEQVLINLLQNALEAIGDSED
GAIQVRCEEAAGGIALTVADNGPGIAADVREELFTPFNTSKEDGLGLGLAISKEIVSDYG
GTIEVESGPSGTTFAVNLKKA