Protein Info for SM_b20458 in Sinorhizobium meliloti 1021

Annotation: dTDP-glucose 4,6-dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K01710, dTDP-glucose 4,6-dehydratase [EC: 4.2.1.46] (inferred from 100% identity to sme:SM_b20458)

MetaCyc: 100% identical to UDP-xylose synthase 1 subunit (Sinorhizobium meliloti 1021)
UDP-glucuronate decarboxylase. [EC: 4.1.1.35]

Predicted SEED Role

"dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 4.2.1.46)

MetaCyc Pathways

O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
UDP-α-D-xylose biosynthesis (2/2 steps found)
dTDP-4-O-demethyl-β-L-noviose biosynthesis (3/5 steps found)
dTDP-β-D-fucofuranose biosynthesis (2/4 steps found)
dTDP-6-deoxy-α-D-allose biosynthesis (2/4 steps found)
dTDP-N-acetylthomosamine biosynthesis (2/4 steps found)
dTDP-N-acetylviosamine biosynthesis (2/4 steps found)
dTDP-L-daunosamine biosynthesis (3/6 steps found)
dTDP-sibirosamine biosynthesis (3/6 steps found)
dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis (5/9 steps found)
dTDP-α-D-mycaminose biosynthesis (2/5 steps found)
dTDP-3-acetamido-α-D-fucose biosynthesis (2/5 steps found)
dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis (2/5 steps found)
dTDP-β-L-digitoxose biosynthesis (3/7 steps found)
dTDP-β-L-olivose biosynthesis (3/7 steps found)
dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis (2/6 steps found)
dTDP-D-desosamine biosynthesis (2/6 steps found)
UDP-sugars interconversion (4/9 steps found)
dTDP-β-L-4-epi-vancosamine biosynthesis (3/8 steps found)
dTDP-β-L-megosamine biosynthesis (3/8 steps found)
dTDP-β-L-mycarose biosynthesis (2/7 steps found)
dTDP-α-D-forosamine biosynthesis (3/9 steps found)
superpathway of enterobacterial common antigen biosynthesis (2/10 steps found)
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (5/19 steps found)
superpathway of novobiocin biosynthesis (4/19 steps found)
superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis (5/23 steps found)
superpathway of erythromycin biosynthesis (2/19 steps found)
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
superpathway of megalomicin A biosynthesis (3/22 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.46

Use Curated BLAST to search for 4.1.1.35 or 4.2.1.46

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92WA4 at UniProt or InterPro

Protein Sequence (348 amino acids)

>SM_b20458 dTDP-glucose 4,6-dehydratase (Sinorhizobium meliloti 1021)
MNYFRNDFRGTLFGTNEATDSLRRRSLQKRILVTGGAGFLGSHLCELLLGAGHEVICLDN
FSTGLRRNIAPLKRFDTFRVIAHDVVEPIDLEVDEIYNLACPASPPHYQADPIQTTKTCV
IGSLNLLDLAARRGARIFQASTSEIYGDPHVHPQVESYWGNVNPFGPRSCYDEGKRCAET
LFFDFHKSHGVEIKIVRIFNTYGPRMRPDDGRVVSNFIVQALKGEDITIYGDGSQTRSFC
FVEDLIDGFVRLMASPPSLTGPVNLGNPAEFTIGELAEEVIRLTGSRSKIVRRPLPVDDP
RQRRPDISLATEELGWRPKVNLAEGLAHTIRYFDDLLSRSMRESAELV