Protein Info for SM_b20342 in Sinorhizobium meliloti 1021

Annotation: isoquinoline 1-oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 737 signal peptide" amino acids 1 to 49 (49 residues), see Phobius details PF20256: MoCoBD_2" amino acids 22 to 215 (194 residues), 49.6 bits, see alignment E=4e-17 amino acids 607 to 686 (80 residues), 52.9 bits, see alignment E=4.1e-18 PF02738: MoCoBD_1" amino acids 330 to 565 (236 residues), 124.7 bits, see alignment E=3.4e-40

Best Hits

KEGG orthology group: K07303, isoquinoline 1-oxidoreductase, beta subunit [EC: 1.3.99.16] (inferred from 100% identity to sme:SM_b20342)

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92WL1 at UniProt or InterPro

Protein Sequence (737 amino acids)

>SM_b20342 isoquinoline 1-oxidoreductase (Sinorhizobium meliloti 1021)
MIPKLMQSITLPVARAEASRRQFLLGALAAGTGVAIGYRILSASPALAGEAATESGSHAF
SPYLTIGGDGKVTVLSSQFEMGQGSYNGIATLVAEELDSDWSAIDVIGAAGNIKAYGNIA
FGGTMQGTGGSTSMSTSWERYRRAGAAARAMLVAAAAAEWGVDAAEIAVENGVLSHPSGK
SGGFGAFAAKAATMPVPADVKLKEPGDWKLIGNDELKRFDSARKANGTEQYTIDVKLPGM
LTAVMIHPPLFGAKAKSFDASAARAIKGVVDVVETPRGIAVVGEHMWAAIKGREAVTVEW
DETGAEKRGTPELMSTYRDLAGKSPAAFARKDGDTDAAFTSAAKVIEATFEFPYLAHAAL
EPLNAVARRNDDGTIEIWGGHQLPDVYQNLASEIAGVPVEKVRLNVMKTGGSFGRRAVFD
GDVVVEAVHVAKALGFRAPVKVQWTREEDTRAGRYRPAYVHRLKAGIDEAGKLVAWSDHI
VGQSIMAKTAWDGMVQNGVDPTSVEGANNLPYAIANQTVGLTTTDVRVPVLWWRSVGSTH
TAFAAEAFLDEVAEAAGRDPLEFRLSMLEPDSRHATVLKLAAEKAEWQKPLPEGRFRGVA
VAESFGSVVAQIAEVSVDGDGIKVERVVAAVDCGLAINPDQVRAQVEGGIGFGLSAILGE
EITLTDGKVDQGNFDMYTPLRIDAMPKVEVHIVASANPPSGIGEPGVPPIGPAVANAAFK
ALGKRIRVMPFAKSLNA