Protein Info for SM_b20198 in Sinorhizobium meliloti 1021

Annotation: ribulose bisophosphate carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02788: RuBisCO_large_N
  • amino acids 27 to 147 (121 residues), 147.9 bits, E=1.6e-47
• PF00016: RuBisCO_large
  • amino acids 157 to 464 (308 residues), 456.9 bits, E=3e-141

Best Hits

Swiss-Prot: 100% identical to RBL1_RHIME: Ribulose bisphosphate carboxylase large chain (cbbL) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01601, ribulose-bisphosphate carboxylase large chain [EC: 4.1.1.39] (inferred from 100% identity to sme:SM_b20198)

MetaCyc: 60% identical to ribulose-bisphosphate carboxylase/oxygenase large subunit (Arabidopsis thaliana col)
Ribulose-bisphosphate carboxylase. [EC: 4.1.1.39]; 1.13.11.- [EC: 4.1.1.39]

Predicted SEED Role

"Ribulose bisphosphate carboxylase large chain (EC 4.1.1.39)" in subsystem CO2 uptake, carboxysome or Calvin-Benson cycle or Carboxysome or Photorespiration (oxidative C2 cycle) (EC 4.1.1.39)

MetaCyc Pathways

• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (22/26 steps found)
• Rubisco shunt (10/10 steps found)
• Calvin-Benson-Bassham cycle (12/13 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• photorespiration I (8/9 steps found)
• photorespiration III (8/9 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• photorespiration II (8/10 steps found)
• oxygenic photosynthesis (13/17 steps found)
• nucleoside and nucleotide degradation (archaea) (4/10 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.39

Use Curated BLAST to search for 4.1.1.39

Sequence Analysis Tools

See P58348 at UniProt or InterPro

Protein Sequence (486 amino acids)

>SM_b20198 ribulose bisophosphate carboxylase (Sinorhizobium meliloti 1021)
MNADAKTEIKGRERYKAGVLKYAQMGYWNGDYEPKDTDLIALFRITPQDGVDPIEAAAAV
AGESSTATWTVVWTDRLTACDQYRAKAYRVDPVPGTPGQYFCYVAYDLILFEEGSIANLT
ASIIGNVFSFKPLKAARLEDMRLPVAYVKTFRGPPTGIVVERERLDKFGKPLLGATTKPK
LGLSGKNYGRVVYEGLKGGLDFMKDDENINSQPFMHWRDRYLYCMEAVNHASAVTGEVKG
HYLNITAGTMEEMYRRAEFAKELGSVIVMVDLIVGWTAIQSISEWCRQNDMILHMHRAGH
GTYTRQKNHGISFRVIAKWLRLAGVDHLHAGTAVGKLEGDPPTVQGYYNVCREMKNEVDL
PRGLFFEQDWADLKKVMPVASGGIHAGQMHQLLDLFGDDVVLQFGGGTIGHPMGIQAGAT
ANRVALEAMVLARNEGRDIAHEGPEILRAAAKWCKPLEAALDIWGNISFNYTPTDTSDFV
PSVTAA