Protein Info for SM_b20197 in Sinorhizobium meliloti 1021

Updated annotation (from data): ribulose-1,5-bisphosphate carboxylase small subunit (EC 4.1.1.39)
Rationale: Specific phenotype: utilization of L-Proline. It appears that proline is oxidized to release CO2 and the CO2 is fixed by Rubisco.
Original annotation: ribulose-1,5-bisphosphate carboxylase small subunit protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to RBS_RHIME: Ribulose bisphosphate carboxylase small chain (cbbS) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01602, ribulose-bisphosphate carboxylase small chain [EC: 4.1.1.39] (inferred from 98% identity to smk:Sinme_3975)

Predicted SEED Role

"Ribulose bisphosphate carboxylase small chain (EC 4.1.1.39)" in subsystem CO2 uptake, carboxysome or Calvin-Benson cycle or Carboxysome or Photorespiration (oxidative C2 cycle) (EC 4.1.1.39)

MetaCyc Pathways

photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (22/26 steps found)
Rubisco shunt (10/10 steps found)
Calvin-Benson-Bassham cycle (12/13 steps found)
1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
ethene biosynthesis V (engineered) (19/25 steps found)
oxygenic photosynthesis (13/17 steps found)
nucleoside and nucleotide degradation (archaea) (4/10 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.39

Use Curated BLAST to search for 4.1.1.39

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P58349 at UniProt or InterPro

Protein Sequence (129 amino acids)

>SM_b20197 ribulose-1,5-bisphosphate carboxylase small subunit (EC 4.1.1.39) (Sinorhizobium meliloti 1021)
MRITQGCFSFLPDLTDEQITAQVQYCLGKGWAIGVEYTDDPHPRNTYWEMWGNPMFDLKD
AKGVMMELEDCRKAHPQDYIRLNAFDSSRGLETVTMSFIVNRPENEPSLRMTRTESNGRS
QHYMWETQR