Protein Info for SGL_RS10130 in Synechocystis sp000284455 PCC 6803

Annotation: fatty acid desaturase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 351 transmembrane" amino acids 45 to 67 (23 residues), see Phobius details amino acids 73 to 90 (18 residues), see Phobius details amino acids 102 to 120 (19 residues), see Phobius details amino acids 167 to 185 (19 residues), see Phobius details amino acids 197 to 220 (24 residues), see Phobius details amino acids 226 to 244 (19 residues), see Phobius details PF00487: FA_desaturase" amino acids 68 to 314 (247 residues), 112.4 bits, see alignment E=1.6e-36

Best Hits

Swiss-Prot: 100% identical to DESA_SYNY3: Delta(12)-fatty-acid desaturase (desA) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K10255, omega-6 fatty acid desaturase (delta-12 desaturase) [EC: 1.14.19.-] (inferred from 100% identity to syn:slr1350)

MetaCyc: 100% identical to sn-1 oleoyl-lipid 12-desaturase (Synechocystis sp. PCC 6803)
RXN-16068 [EC: 1.14.19.45]

Predicted SEED Role

"phosphatidylcholine desaturase"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.19.-

Use Curated BLAST to search for 1.14.19.- or 1.14.19.45

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (351 amino acids)

>SGL_RS10130 fatty acid desaturase (Synechocystis sp000284455 PCC 6803)
MTATIPPLTPTVTPSNPDRPIADLKLQDIIKTLPKECFEKKASKAWASVLITLGAIAVGY
LGIIYLPWYCLPITWIWTGTALTGAFVVGHDCGHRSFAKKRWVNDLVGHIAFAPLIYPFH
SWRLLHDHHHLHTNKIEVDNAWDPWSVEAFQASPAIVRLFYRAIRGPFWWTGSIFHWSLM
HFKLSNFAQRDRNKVKLSIAVVFLFAAIAFPALIITTGVWGFVKFWLMPWLVYHFWMSTF
TIVHHTIPEIRFRPAADWSAAEAQLNGTVHCDYPRWVEVLCHDINVHIPHHLSVAIPSYN
LRLAHGSLKENWGPFLYERTFNWQLMQQISGQCHLYDPEHGYRTFGSLKKV