Protein Info for SGL_RS06815 in Synechocystis sp000284455 PCC 6803

Annotation: Re/Si-specific NAD(P)(+) transhydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 530 transmembrane" amino acids 189 to 209 (21 residues), see Phobius details amino acids 422 to 439 (18 residues), see Phobius details amino acids 445 to 465 (21 residues), see Phobius details amino acids 472 to 493 (22 residues), see Phobius details amino acids 501 to 520 (20 residues), see Phobius details TIGR00561: NAD(P)(+) transhydrogenase (AB-specific), alpha subunit" amino acids 21 to 530 (510 residues), 994.6 bits, see alignment E=4.7e-304 PF05222: AlaDh_PNT_N" amino acids 23 to 158 (136 residues), 158.5 bits, see alignment E=1.7e-50 PF01262: AlaDh_PNT_C" amino acids 162 to 386 (225 residues), 242.2 bits, see alignment E=6.1e-76 PF12769: PNTB_4TM" amino acids 446 to 530 (85 residues), 122 bits, see alignment E=1.8e-39

Best Hits

Swiss-Prot: 58% identical to PNTA_RHOSH: NAD(P) transhydrogenase subunit alpha (pntA) from Rhodobacter sphaeroides

KEGG orthology group: K00324, NAD(P) transhydrogenase subunit alpha [EC: 1.6.1.2] (inferred from 100% identity to syn:slr1239)

Predicted SEED Role

"NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2)" in subsystem Phosphate metabolism (EC 1.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.1.2

Use Curated BLAST to search for 1.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (530 amino acids)

>SGL_RS06815 Re/Si-specific NAD(P)(+) transhydrogenase subunit alpha (Synechocystis sp000284455 PCC 6803)
MTIAAPKTTVAPEMKPTTPKKIGVPRESFDQECRVAMTPDTAQKLQKLGFDLLLETGAGA
KADFADRLYESLGCGIVDGRQTLFEQADIILKVRPPSPAEVEELPAGKALISFIWPAQNP
ELLEKLAAKNITVLAMDAVPRISRAQKLDALSSMANIAGYRAIIEAANRFGRFFTGQITA
AGKVPPAKVLVIGAGVAGLAAIGAAKSLGAIVRAFDTRPVVKEQVESLGGEFLLLDFKED
GTGQGGYAKVMSEEFIAAEMALFAAQAKEVDIIITTALIPGRPAPKLITEPMVKSMRAGS
VVVDLAAEQGGNCEVTKPGEVYEYEGVTIIGYTDLPSRMASQSSQLYGTNLWHLLKDMGG
SEAFKVDVEDEVIRGALVSHEGKITWPPPKLNPPSPVQPAKPTVTVVESEAKSAKKSGSG
NFLWLALAAIALVGIGIGAPTSFLSHFTVFVLACVVGWQLIWNVAPALHTPLMSVTNAIS
GIIIIGGMLQISGPLNSPTTILGAIAILVGTINIAGGFLVTQRMLKMFRK