Protein Info for SGL_RS03595 in Synechocystis sp000284455 PCC 6803

Annotation: NAD-dependent epimerase/dehydratase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 129 to 367 (239 residues), 68.8 bits, E=1.1e-22
• PF01370: Epimerase
  • amino acids 130 to 333 (204 residues), 95.2 bits, E=1e-30
• PF01073: 3Beta_HSD
  • amino acids 132 to 295 (164 residues), 26.2 bits, E=9.7e-10
• PF07993: NAD_binding_4
  • amino acids 178 to 321 (144 residues), 22.1 bits, E=1.9e-08
• PF16363: GDP_Man_Dehyd
  • amino acids 186 to 402 (217 residues), 59.7 bits, E=9.1e-20

Best Hits

KEGG orthology group: None (inferred from 100% identity to syn:slr1617)

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (10/11 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (3/6 steps found)
• D-galactose degradation I (Leloir pathway) (2/5 steps found)
• UDP-sugars interconversion (3/9 steps found)
• stachyose degradation (1/7 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2

Protein Sequence (411 amino acids)

>SGL_RS03595 NAD-dependent epimerase/dehydratase family protein (Synechocystis sp000284455 PCC 6803)
MKIRWITPLLGTAAFNAVQNITDITVVDVRDLVDKAGNDNSAILHKIHQGVDLIKQGKRT
VICCDYGMSRSNAIAVGIITNLEKIPFNQALRQVQEATGEAEIKLDPLNAVRQALGVNIK
KEKCSQYRNILITGGRGFIGTALQGALINSEFRLISPTREQIDIFAGSTKLDLLASEENI
DCIVHLANPRVYTSNVAMGQTLTMLRNVIDVCLAKDIPLIYPSSWEIYSGYAGTIHADES
TPALPRGPYGETKYLAEILIDHCRRTRGLRCAILRSSPVYGSMSDKPKFIFNFFKKASQG
QKIVTHHYINGNPKLDLLHIDDLISSIVATLKSRFIGNLNIGTGQLSSTLKIAEMIRDEL
GSSSMIQQIEVNTEVASIAMNYGRANHVLDWEPVIFFEQGLKSLLHQISTN