Protein Info for Rv3452 in Mycobacterium tuberculosis H37Rv

Annotation: Probable cutinase precursor Cut4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 52% identical to CUT2_MYCTU: Probable cutinase cut2 (cut2) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K01066, esterase / lipase [EC: 3.1.1.-] (inferred from 100% identity to mbt:JTY_3518)

Predicted SEED Role

"Probable cutinase Cut4 (EC 3.1.1.74)" (EC 3.1.1.74)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.-, 3.1.1.74

Use Curated BLAST to search for 3.1.1.- or 3.1.1.74

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (226 amino acids)

>Rv3452 Probable cutinase precursor Cut4 (Mycobacterium tuberculosis H37Rv)
VIPRPQPHSGRWRAGAARRLTSLVAAAFAAATLLLTPALAPPASAGCPDAEVVFARGTGE
PPGLGRVGQAFVSSLRQQTNKSIGTYGVNYPANGDFLAAADGANDASDHIQQMASACRAT
RLVLGGYSQGAAVIDIVTAAPLPGLGFTQPLPPAADDHIAAIALFGNPSGRAGGLMSALT
PQFGSKTINLCNNGDPICSDGNRWRAHLGYVPGMTNQAARFVASRI