Protein Info for Rv3409c in Mycobacterium tuberculosis H37Rv

Annotation: Cholesterol oxidase ChoD (cholesterol-O2 oxidoreductase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 578 PF00890: FAD_binding_2" amino acids 6 to 39 (34 residues), 20.6 bits, see alignment (E = 5.7e-08) PF13450: NAD_binding_8" amino acids 9 to 39 (31 residues), 23.8 bits, see alignment (E = 1.1e-08) PF00732: GMC_oxred_N" amino acids 190 to 277 (88 residues), 23.8 bits, see alignment E=9.1e-09 PF05199: GMC_oxred_C" amino acids 468 to 522 (55 residues), 42.4 bits, see alignment 2.8e-14

Best Hits

Swiss-Prot: 100% identical to CHOD_MYCTU: Cholesterol oxidase (choD) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K03333, cholesterol oxidase [EC: 1.1.3.6] (inferred from 100% identity to mtb:TBMG_03460)

MetaCyc: 100% identical to cholesterol oxidase (Mycobacterium tuberculosis H37Rv)
CHOLESTEROL-OXIDASE-RXN

Predicted SEED Role

"Cholesterol oxidase (EC 1.1.3.6)" (EC 1.1.3.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.3.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (578 amino acids)

>Rv3409c Cholesterol oxidase ChoD (cholesterol-O2 oxidoreductase) (Mycobacterium tuberculosis H37Rv)
MKPDYDVLIIGSGFGGSVTALRLTEKGYRVGVLEAGRRFSDEEFAKTSWDLRKFLWAPRL
GCYGIQRIHPLRNVMILAGAGVGGGSLNYANTLYVPPEPFFADQQWSHITDWRGELMPHY
QQAQRMLGVVQNPTFTDADRIVKEVADEMGFGDTWVPTPVGVFFGPDGTKTPGKTVPDPY
FGGAGPARTGCLECGCCMTGCRHGAKNTLVKNYLGLAESAGAQVIPMTTVKGFERRSDGL
WEVRTVRTGSWLRRDRRTFTATQLVLAAGTWGTQHLLFKMRDRGRLPGLSKRLGVLTRTN
SESIVGAATLKVNPDLDLTHGVAITSSIHPTADTHIEPVRYGKGSNAMGLLQTLMTDGSG
PQGTDVPRWRQLLQTASQDPRGTIRMLNPRQWSERTVIALVMQHLDNSITTFTKRGKLGI
RWYSSKQGHGEPNPTWIPIGNQVTRRIAAKIDGVAGGTWGELFNIPLTAHFLGGAVIGDD
PEHGVIDPYHRVYGYPTLYVVDGAAISANLGVNPSLSIAAQAERAASLWPNKGETDRRPP
QGEPYRRLAPIQPAHPVVPADAPGALRWLPIDPVSNAG