Protein Info for Rv2444c in Mycobacterium tuberculosis H37Rv

Annotation: Possible ribonuclease E Rne

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 953 TIGR00757: ribonuclease, Rne/Rng family" amino acids 351 to 766 (416 residues), 527.2 bits, see alignment E=1.5e-162 PF10150: RNase_E_G" amino acids 455 to 736 (282 residues), 320.3 bits, see alignment E=5.3e-100

Best Hits

Swiss-Prot: 65% identical to RNE_MYCS2: Ribonuclease E (rne) from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

KEGG orthology group: K08300, ribonuclease E [EC: 3.1.26.12] (inferred from 99% identity to mbo:Mb2471c)

Predicted SEED Role

"Ribonuclease E (EC 3.1.26.12)" in subsystem RNA processing and degradation, bacterial or Ribosome biogenesis bacterial (EC 3.1.26.12)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.26.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (953 amino acids)

>Rv2444c Possible ribonuclease E Rne (Mycobacterium tuberculosis H37Rv)
VIDGAPPSDPPEPSQHEELPDRLRVHSLARTLGTTSRRVLDALTALDGRVRSAHSTVDRV
DAVRVRDLLATHLETAGVLAASVHAPEASEEPESRLMLETQETRNADVERPHYMPLFVAP
QPIPEPLADDEDVDDGPDYVADDSDADDEGQLDRPANRRRRRGRRGRGRGRGEQGGSDGD
PVDQQSEPRAQQFTSADAAETDDGDDRDSEDTEAGDNGEDENGSLEAGNRRRRRRRRRKS
ASGDDNDAALEGPLPDDPPNTVVHERVPRAGDKAGNSQDGGSGSTEIKGIDGSTRLEAKR
QRRRDGRDAGRRRPPVLSEAEFLARREAVERVMVVRDRVRTEPPLPGTRYTQIAVLEDGI
VVEHFVTSAASASLVGNIYLGIVQNVLPSMEAAFVDIGRGRNGVLYAGEVNWDAAGLGGA
DRKIEQALKPGDYVVVQVSKDPVGHKGARLTTQVSLAGRFLVYVPGASSTGISRKLPDTE
RQRLKEILREVVPSDAGVIIRTASEGVKEDDIRADVARLRERWEQIEAKAQETKEKAAGA
AVALYEEPDVLVKVIRDLFNEDFVGLIVSGDEAWNTINEYVNSVAPELVSKLTKYESADG
PDGQSAPDVFTVHRIDEQLAKAMDRKVWLPSGGTLVIDRTEAMTVIDVNTGKFTGAGGNL
EQTVTKNNLEAAEEIVRQLRLRDIGGIVVIDFIDMVLESNRDLVLRRLTESLARDRTRHQ
VSEVTSLGLVQLTRKRLGTGLIEAFSTSCPNCSGRGILLHADPVDSAAATGRKSEPGARR
GKRSKKSRSEESSDRSMVAKVPVHAPGEHPMFKAMAAGLSSLAGRGDEESGEPAAELAEQ
AGDQPPTDLDDTAQADFEDTEDTDEDEDELDADEDLEDLDDEDLDEDLDVEDSDSDDEDS
DEDAADADVDEEDAAGLDGSPGEVDVPGVTELAPTRPRRRVAGRPAGPPIRLD