Protein Info for Rv2357c in Mycobacterium tuberculosis H37Rv

Annotation: Probable glycyl-tRNA synthetase GlyS (glycine--tRNA ligase) (GLYRS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 TIGR00389: glycine--tRNA ligase" amino acids 10 to 239 (230 residues), 263.4 bits, see alignment E=2.1e-82 PF00587: tRNA-synt_2b" amino acids 160 to 331 (172 residues), 35.8 bits, see alignment E=9.1e-13 PF03129: HGTP_anticodon" amino acids 369 to 457 (89 residues), 68.6 bits, see alignment E=4.4e-23

Best Hits

Swiss-Prot: 100% identical to SYG_MYCTU: Glycine--tRNA ligase (glyQS) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K01880, glycyl-tRNA synthetase [EC: 6.1.1.14] (inferred from 100% identity to mra:MRA_2381)

Predicted SEED Role

"Glycyl-tRNA synthetase (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (463 amino acids)

>Rv2357c Probable glycyl-tRNA synthetase GlyS (glycine--tRNA ligase) (GLYRS) (Mycobacterium tuberculosis H37Rv)
VHHPVAPVIDTVVNLAKRRGFVYPSGEIYGGTKSAWDYGPLGVELKENIKRQWWRSVVTG
RDDVVGIDSSIILPREVWVASGHVDVFHDPLVESLITHKRYRADHLIEAYEAKHGHPPPN
GLADIRDPETGEPGQWTQPREFNMMLKTYLGPIETEEGLHYLRPETAQGIFVNFANVVTT
ARKKPPFGIGQIGKSFRNEITPGNFIFRTREFEQMEMEFFVEPATAKEWHQYWIDNRLQW
YIDLGIRRENLRLWEHPKDKLSHYSDRTVDIEYKFGFMGNPWGELEGVANRTDFDLSTHA
RHSGVDLSFYDQINDVRYTPYVIEPAAGLTRSFMAFLIDAYTEDEAPNTKGGMDKRTVLR
LDPRLAPVKAAVLPLSRHADLSPKARDLGAELRKCWNIDFDDAGAIGRRYRRQDEVGTPF
CVTVDFDSLQDNAVTVRERDAMTQDRVAMSSVADYLAVRLKGS