Protein Info for Rv2291 in Mycobacterium tuberculosis H37Rv

Annotation: Probable thiosulfate sulfurtransferase SseB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00581: Rhodanese
  • amino acids 10 to 131 (122 residues), 60.8 bits, E=7.5e-21
  • amino acids 166 to 273 (108 residues), 42.5 bits, E=3.9e-15

Best Hits

Swiss-Prot: 100% identical to THT3_MYCTO: Putative thiosulfate sulfurtransferase SseB (sseB) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K01011, thiosulfate/3-mercaptopyruvate sulfurtransferase [EC: 2.8.1.1 2.8.1.2] (inferred from 99% identity to mbb:BCG_2308)

Predicted SEED Role

"Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1)" (EC 2.8.1.1)

MetaCyc Pathways

• thiosulfate disproportionation IV (rhodanese) (1/1 steps found)
• L-cysteine degradation III (1/2 steps found)
• sulfide oxidation IV (mitochondria) (2/5 steps found)

KEGG Metabolic Maps

• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.1, 2.8.1.2

Use Curated BLAST to search for 2.8.1.1 or 2.8.1.2

Protein Sequence (284 amino acids)

>Rv2291 Probable thiosulfate sulfurtransferase SseB (Mycobacterium tuberculosis H37Rv)
VQARGQVLITAAELAGMIQAGDPVSILDVRWRLDEPDGHAAYLQGHLPGAVFVSLEDELS
DHTIAGRGRHPLPSGASLQATVRRCGIRHDVPVVVYDDWNRAGSARAWWVLTAAGIANVR
ILDGGLPAWRSAGGSIETGQVSPQLGNVTVLHDDLYAGQRLTLTAQQAGAGGVTLLDARV
PERFRGDVEPVDAVAGHIPGAINVPSGSVLADDGTFLGNGALNALLSDHGIDHGGRVGVY
CGSGVSAAVIVAALAVIGQDAELFPGSWSEWSSDPTRPVGRGTA