Protein Info for Rv2196 in Mycobacterium tuberculosis H37Rv

Annotation: Probable ubiquinol-cytochrome C reductase QcrB (cytochrome B subunit)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 549 transmembrane" amino acids 43 to 69 (27 residues), see Phobius details amino acids 114 to 134 (21 residues), see Phobius details amino acids 146 to 166 (21 residues), see Phobius details amino acids 176 to 199 (24 residues), see Phobius details amino acids 212 to 237 (26 residues), see Phobius details amino acids 261 to 287 (27 residues), see Phobius details amino acids 334 to 355 (22 residues), see Phobius details amino acids 375 to 398 (24 residues), see Phobius details amino acids 414 to 434 (21 residues), see Phobius details PF00033: Cytochrome_B" amino acids 45 to 235 (191 residues), 82.8 bits, see alignment E=2.9e-27 PF13631: Cytochrom_B_N_2" amino acids 120 to 294 (175 residues), 218.1 bits, see alignment E=7.8e-69

Best Hits

Swiss-Prot: 100% identical to QCRB_MYCTU: Cytochrome bc1 complex cytochrome b subunit (qcrB) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K03891, ubiquinol-cytochrome c reductase cytochrome b subunit (inferred from 100% identity to mbo:Mb2219)

Predicted SEED Role

"Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2)" in subsystem Ubiquinone Menaquinone-cytochrome c reductase complexes (EC 1.10.2.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.10.2.2

Use Curated BLAST to search for 1.10.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (549 amino acids)

>Rv2196 Probable ubiquinol-cytochrome C reductase QcrB (cytochrome B subunit) (Mycobacterium tuberculosis H37Rv)
MSPKLSPPNIGEVLARQAEDIDTRYHPSAALRRQLNKVFPTHWSFLLGEIALYSFVVLLI
TGVYLTLFFDPSMVDVTYNGVYQPLRGVEMSRAYQSALDISFEVRGGLFVRQIHHWAALM
FAAAIMVHLARIFFTGAFRRPRETNWVIGSLLLILAMFEGYFGYSLPDDLLSGLGLRAAL
SSITLGMPVIGTWLHWALFGGDFPGTILIPRLYALHILLLPGIILALIGLHLALVWFQKH
TQFPGPGRTEHNVVGVRVMPVFAFKSGAFFAAIVGVLGLMGGLLQINPIWNLGPYKPSQV
SAGSQPDFYMMWTEGLARIWPPWEFYFWHHTIPAPVWVAVIMGLVFVLLPAYPFLEKRFT
GDYAHHNLLQRPRDVPVRTAIGAMAIAFYMVLTLAAMNDIIALKFHISLNATTWIGRIGM
VILPPFVYFITYRWCIGLQRSDRSVLEHGVETGIIKRLPHGAYIELHQPLGPVDEHGHPI
PLQYQGAPLPKRMNKLGSAGSPGSGSFLFADSAAEDAALREAGHAAEQRALAALREHQDS
IMGSPDGEH