Protein Info for Rv1844c in Mycobacterium tuberculosis H37Rv

Annotation: Probable 6-phosphogluconate dehydrogenase Gnd1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00873: 6-phosphogluconate dehydrogenase (decarboxylating)
  • amino acids 12 to 477 (466 residues), 727.2 bits, E=4.2e-223
• PF03446: NAD_binding_2
  • amino acids 12 to 183 (172 residues), 157.4 bits, E=5e-50
• PF00393: 6PGD
  • amino acids 188 to 476 (289 residues), 418.4 bits, E=2.5e-129

Best Hits

Swiss-Prot: 56% identical to 6PGD_BACSU: 6-phosphogluconate dehydrogenase, NADP(+)-dependent, decarboxylating (gndA) from Bacillus subtilis (strain 168)

KEGG orthology group: K00033, 6-phosphogluconate dehydrogenase [EC: 1.1.1.44] (inferred from 100% identity to mra:MRA_1855)

MetaCyc: 56% identical to 6-phosphogluconate dehydrogenase (decarboxylating) (Bacillus subtilis)
Phosphogluconate dehydrogenase (decarboxylating). [EC: 1.1.1.44]

Predicted SEED Role

"6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)" in subsystem Pentose phosphate pathway (EC 1.1.1.44)

MetaCyc Pathways

• pentose phosphate pathway (8/8 steps found)
• superpathway of glucose and xylose degradation (14/17 steps found)
• pentose phosphate pathway (oxidative branch) I (3/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Glutathione metabolism
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.44

Use Curated BLAST to search for 1.1.1.44

Protein Sequence (485 amino acids)

>Rv1844c Probable 6-phosphogluconate dehydrogenase Gnd1 (Mycobacterium tuberculosis H37Rv)
MSSSESPAGIAQIGVTGLAVMGSNIARNFARHGYTVAVHNRSVAKTDALLKEHSSDGKFV
RSETIPEFLAALEKPRRVLIMVKAGEATDADAVINELADAMEPGDIIIDGGNALYTDTMR
REKAMRERGLHFVGAGISGGEEGALNGPSIMPGGPAESYQSLGPLLEEISAHVDGVPCCT
HIGPDGSGHFVKMVHNGIEYSDMQLIGEAYQLMRDGLGLTAPAIADVFTEWNNGDLDSYL
VEITAEVLRQTDAKTGKPLVDVIVDRAEQKGTGRWTVKSALDLGVPVTGIAEAVFARALS
GSVGQRSAASGLASGKLGEQPADPATFTEDVRQALYASKIVAYAQGFNQIQAGSAEFGWD
ITPGDLATIWRGGCIIRAKFLNHIKEAFDASPNLASLIVAPYFRGAVESAIDSWRRVVST
AAQLGIPTPGFSSALSYYDALRTARLPAALTQAQRDFFGAHTYGRIDEPGKFHTLWSSDR
TEVPV